Rat anti Mouse GITR/CD357 Antibody, Monoclonal (DTA-1), could be used for Flow Cytometry.
规格
产品名称
GITR/CD357 Rat mAb (PE)
别名
糖皮质激素诱导肿瘤坏死因子受体抗体 | GITR/CD357抗体
英文别名
Gitr | Activation-inducible TNFR family receptor | AITR | AITRTNF receptor superfamily activation-inducible protein | CD357 antigen | CD357 | GITR-D | GITRtumor necrosis factor receptor superfamily member 18 | Glucocorticoid-induced TNFR-related protein |
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
生化机理
Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
宿主种属
大鼠(Rat)
特异性
GITR/CD357
种属反应性
小鼠(Mouse)
免疫原
Mouse CD25+ CD4+ T cells
阳性对照
Flow: C57BL/6 mouse splenocytes.
偶联
PE
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
lambda
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
GITR/CD357 Rat mAb (PE) (Ab190291) - Flow Cytometry Two-color flow cytometric analysis of GITR/CD357 expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD25 Rat mAb (AF647) (Ab211659) and either Rat IgG (PE) (Ab180312; Left panel) or GITR/CD357 Rat mAb (PE) (Ab190291; Right panel) at 0.3 μg/test.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.