Mouse anti Mouse MHC class I/H-2Kb Antibody, Monoclonal (AF6-88.5.5.3), could be used for Flow Cytometry.
规格
产品名称
MHC class I/H-2Kb Mouse mAb (AF488)
别名
组织相容性复合体蛋白1抗体 | MHC class I/H-2Kb抗体
英文别名
K-f | H-2K | H2-K | H2-D1 | H-2K(d) | A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alpha chain | HLA Class I
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
宿主种属
小鼠(Mouse)
特异性
MHC class I/H-2Kb
种属反应性
小鼠(Mouse)
免疫原
C57BL/6 mouse splenocytes
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes.
偶联
AF488
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
MHC class I/H-2Kb Mouse mAb (AF488) (Ab190296) - Flow Cytometry Flow cytometry analysis of MHC class I/H-2Kb expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with MHC class I/H-2Kb Mouse mAb (AF488) (Ab190296) at 0.3μg/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).
MHC class I/H-2Kb Mouse mAb (AF488) (Ab190296) - Flow Cytometry Flow cytometry analysis of MHC class I/H-2Kb expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with MHC class I/H-2Kb Mouse mAb (AF488) (Ab190296) at 0.3μg/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.