The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis..
宿主种属
小鼠(Mouse)
特异性
BrdU
种属反应性
不依赖种属(Species independent)
免疫原
Bromodeoxyuridine
阳性对照
IF/ICC: HeLa cells
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
lambda
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.4
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
图片
Recombinant BrdU Antibody (Ab210601) - IF/ICC IF analysis of Recombinant BrdU Antibody (Green) in HeLa cells. The cells were fixed with ethanol for 15 minutes and subjected to acid hydrolysis using 2M HCl for 15 minutes at room temperature to denature the DNA. They were then stained Recombinant BrdU Antibody (Ab210601) at 1.0 μg/mL in blocking buffer overnight at 4℃ and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
ZJ25F0825666
危险品化学品经营许可证（带存储）