Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G (PubMed:25920998).
宿主种属
大鼠(Rat)
特异性
CD3
种属反应性
小鼠(Mouse)
免疫原
γδTCR-positive T-T hybridoma D1
阳性对照
Flow: Mouse splenocytes.
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein G purified
浓度
见 COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
10mM PBS, pH 7.2-7.4
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
图片
Recombinant CD3 Antibody (Ab210605) - Flow Cytometry Flow Cytometry analysis of C57BL/6 mouse splenocytes labelling CD3 with Recombinant CD3 Antibody (Ab210605) conjugated with biotin (right panel) at 1.0 μg/mL for 1 hour at 4°C, compared with Rat IgG (Ab180310) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).
Recombinant CD3 Antibody (Ab210605) - Flow Cytometry Flow Cytometry analysis of C57BL/6 mouse splenocytes labelling CD3 (red) with Recombinant CD3 Antibody (Ab210605) conjugated with biotin at 1.0 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Rat IgG (Ab180310) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD3 Antibody (Ab210605) - SEC The purity of Recombinant CD3 Antibody (Ab210605) is more than 95% verified by HPLC.
ZJ25F0927029
危险品化学品经营许可证（带存储）