Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways.
In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Also participates in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.
In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx.
宿主种属
小鼠(Mouse)
特异性
Human CD3ε
种属反应性
人(Human)
免疫原
Human thymocytes
阳性对照
Flow Cytometry: Human peripheral blood lymphocytes
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 6.0
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融.
图片
Recombinant CD3 Antibody (Ab210610) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphcytes labelling CD3 with Recombinant CD3 Antibody (Ab210610) (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Ab170221) (left panel) and detected with AF 488-conjugated affinipure goat anti-Mouse IgG.
Recombinant CD3 Antibody (Ab210610) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphcytes labelling CD3 (red) with Recombinant CD3 Antibody (Ab210610) at 0.2 μg/mL for 1 hour at 4°C, and detected with AF 488-conjugated affinipure goat anti-Mouse IgG. Blue - Mouse IgG Isotype Control Antibody (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD3 Antibody (Ab210610) - SEC The purity of Recombinant CD3 Antibody (Ab210610) is more than 95% verified by HPLC.
ZJ25F0927360
危险品化学品经营许可证（带存储）