Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
宿主种属
大鼠(Rat)
特异性
Mouse c-Kit
种属反应性
小鼠(Mouse)
免疫原
Mouse bone marrow mast cells
阳性对照
Flow Cytometry: Mouse bone marrow cells
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.4
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
图片
Recombinant c-Kit Antibody (Ab210970) - Flow Cytometry Two-color flow cytometric analysis of c-Kit expression on mouse bone marrow. C57BL/6 mouse bone marrow cells were stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or Recombinant c-Kit Antibody (Ab210970; Right panel) at 1.0 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD45R versus c-Kit (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.
Recombinant c-Kit Antibody (Ab210970) - SEC The purity of Recombinant c-Kit Antibody (Ab210970) is more than 95% verified by HPLC.
ZJ25F0926787
危险品化学品经营许可证（带存储）