Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration (By similarity).
宿主种属
大鼠(Rat)
特异性
Mouse CD146
种属反应性
小鼠(Mouse)
免疫原
Endothelial cell line TME-3H3
阳性对照
Flow Cytometry: C57BL/6 splenocytes
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.4
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
图片
Recombinant CD146 Antibody (Ab211671) - Flow Cytometry Flow Cytometry analysis of bEnd.3 labelling CD146 with Recombinant CD146 Antibody (Ab211671) conjugated with biotin (right panel) at 0.2 μg/mL for 1 hour at 4°C, compared with Rat IgG (Ab180310) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).
Recombinant CD146 Antibody (Ab211671) - Flow Cytometry Flow Cytometry analysis of bEnd.3 labelling CD146 (red) with Recombinant CD146 Antibody (Ab211671) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Rat IgG (Ab180310) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD146 Antibody (Ab211671) - Flow Cytometry Two-color flow cytometric analysis of CD146 expression on mouse splenocytes. C57BL/6 mouse splenocytes were stained with NKG2A/C/E Rat mAb (FITC) (Ab191686) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or Recombinant CD146 Antibody (Ab211671; Right panel) at 0.2 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of NKG2A/C/E versus CD146 (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.
Recombinant CD146 Antibody (Ab211671) - SEC The purity of Recombinant CD146 Antibody (Ab211671) is more than 95% verified by HPLC.
ZJ25F1029050
危险品化学品经营许可证（带存储）