Recombinant CD45 Antibody, clone P07H10, could be used for Flow Cytometry.
规格
产品名称
Recombinant CD45 Antibody (PE)
别名
CD45抗体
英文别名
B220 antibody | CD 45 antibody | CD45 antibody | CD45 antigen antibody | CD45R antibody | GP180 antibody | L-CA antibody | LCA antibody | Leukocyte common antigen antibody | loc antibody | Ly-5 antibody | LY5 antibody | Ly5, homolog of antibody | Lyt-4 an
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
生化机理
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity).
Dephosphorylates LYN, and thereby modulates LYN activity.
宿主种属
大鼠(Rat)
特异性
Mouse CD45
种属反应性
小鼠(Mouse)
免疫原
Mouse thymus or spleen
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
PE
Excitation(nm)
Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)
575nm
Ex/Em(nm)
Ex:565nm;Em:575nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant CD45 Antibody (PE) (Ab213885) - Flow Cytometry Flow cytometry analysis of CD45 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant CD45 Antibody (PE) (Ab213885) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).
Recombinant CD45 Antibody (PE) (Ab213885) - Flow Cytometry Flow cytometry analysis of CD45 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant CD45 Antibody (PE) (Ab213885) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤0.3μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.
ZJ25F0318943
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