CD16: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b.
Mediates neutrophil activation by IgG complexes redundantly with Fcgr4.
CD32: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin-coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B-cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.
宿主种属
大鼠(Rat)
特异性
Mouse CD16/CD32
种属反应性
小鼠(Mouse)
免疫原
Mouse BALB/c Macrophage J774
阳性对照
Flow Cytometry: C57BL/6 mouse splenocytes
偶联
AF488
Excitation(nm)
Blue Laser(488nm)
Emission(nm)
525nm
Ex/Em(nm)
Ex:490nm;Em:525nm
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
lambda
纯化方法
Protein A purified
浓度
0.5mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
2-8°C储存,避光,禁止冷冻
运输条件
低温运输,禁止冷冻
稳定性与储存
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
图片
Recombinant CD16/CD32 Antibody (AF488) (Ab223915) - Flow Cytometry Flow cytometry analysis of CD16/CD32 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD16/CD32 Antibody (AF488) (Ab223915) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF488) (Ab190240).
Recombinant CD16/CD32 Antibody (AF488) (Ab223915) - Flow Cytometry Flow cytometry analysis of CD16/CD32 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD16/CD32 Antibody (AF488) (Ab223915) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (AF488) (Ab190240). Black - Unlabelled control, cells without incubation with primary antibody.
应用
应用名称
稀释比例
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Flow Cytometry
Use at an assay dependent concentration. Each lot of this antibody is quality control tested by flow cytometric analysis as negative control. Use at concentrations comparable to those of the speci
ZJ25F1028671
危险品化学品经营许可证（带存储）