Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D.
宿主种属
小鼠(Mouse)
特异性
Human DNA PKcs
种属反应性
人(Human)
免疫原
Recombinant protein corresponding to human DNA PKcs
阳性对照
Flow Cytometry: HepG2 cells
偶联
Unconjugated
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.4
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融.
图片
DNA PKcs Mouse mAb (Ab214908) - Flow Cytometry Overlay histogram showing HepG2 cells stained with DNA PKcs Mouse mAb (Ab214908) (Red) at 0.25 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG1-kappa, anti-E8L (Ab213557) was used as an isotype control (Blue).
ZJ25F0825627
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