Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.
The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
宿主种属
小鼠(Mouse)
特异性
Human 14-3-3 beta/alpha
种属反应性
人(Human)
免疫原
Recombinant protein corresponding to Human 14-3-3 beta/alpha
阳性对照
WB: HeLa; MCF-7; HEK293; HT-29
偶联
Unconjugated
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.2
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
14-3-3 beta/alpha Mouse mAb (Ab215196) - Western Blot
All lanes: 14-3-3 beta/alpha Mouse mAb (Ab215196) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
ZJ26F0535403
危险品化学品经营许可证（带存储）