Mouse anti His tag (C-terminal) Antibody, clone IJ05/3D2, could be used for WB, ELISA and so on.
规格
产品名称
His tag (C-terminal) Mouse mAb
别名
聚组氨酸标签单克隆抗体 | 聚组氨酸抗体 | His tag标签抗体 | His tag单克隆抗体 | 6xHis抗体 | His标签 | His标签抗体 | His标签小鼠单克隆抗体 | His抗体.
英文别名
6 His epitope tag antibody | Hexa His tag antibody | HHHHHH epitope tag antibody | HHHHHH tag antibody | His tag antibody | Polyhistidine Tag antibody | His tag Mouse Monoclonal Antibody | His tag Mouse mAb | Anti-His tag | Monoclonal Anti- His tag antibo
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
宿主种属
小鼠(Mouse)
特异性
Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
种属反应性
不依赖种属(Species independent)
免疫原
A recombinant protein containing the sequence HHHHHH
阳性对照
WB: His-transfected HEK293 whole cell lysates; C-terminal His fusion proteins
偶联
Unconjugated
备注
此产品可替代 Ab086831、Ab155835、Ab219141、Ab219147
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.2
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。
图片
His tag (C-terminal) Mouse mAb (Ab219145) - Western Blot All lanes: His tag (C-terminal) Mouse Antibody (Ab219145) at 1/10000 dilution Samples: Lysates at 10 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 48,116 kDa Observed band size: 52,118 kDa Exposure time: 38.3 s
His tag (C-terminal) Mouse mAb (Ab219145) - Western Blot All lanes: His tag (C-terminal) Mouse Antibody (Ab219145) at 1/10000 dilution Samples: Lysates at 10 ng per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 35,70 kDa Observed band size: 34,67 kDa Exposure time: 37.6 s
ZJ25F0521249
危险品化学品经营许可证（带存储）