Mouse anti Human GAPDH Antibody, Monoclonal (B08/4A11), could be used for IF/ICC and Flow Cytometry.
规格
产品名称
GAPDH Mouse mAb (AF555)
别名
重组Anti-GAPDH抗体 | 小鼠单克隆抗体 to GAPDH
英文别名
38 kDa BFA-dependent ADP-ribosylation substrate antibody | aging associated gene 9 protein antibody | Aging-associated gene 9 protein antibody | BARS-38 antibody | cb609 antibody | EC 1.2.1.12 antibody | Epididymis secretory sperm binding protein Li 162eP
规格或纯度
ExactAb™, 已验证, 0.5 mg/mL
宿主种属
小鼠(Mouse)
特异性
GAPDH
种属反应性
人(Human)
免疫原
Recombinant full length human GAPDH
阳性对照
Flow Cytometry: Hela cells IF/ICC: MCF-7 cells
偶联
AF555
Excitation(nm)
Green Laser(532nm)
Emission(nm)
568nm
Ex/Em(nm)
Ex:553nm;Em:568nm
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂
0.05% Proclin300
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
图片
GAPDH Mouse mAb (AF555) (Ab219257) – IF/ICC IF analysis of GAPDH (red) in MCF-7 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes and permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were then stained with GAPDH Mouse mAb (AF555) (Ab219257) at a dilution of 1/1000 in blocking buffer for 2 hour at RT. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
GAPDH Mouse mAb (AF555) (Ab219257) - Flow Cytometry Flow cytometry analysis of GAPDH expression by Hela cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with GAPDH Mouse mAb (AF555) (Ab219257) (red) at 1/450 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF555) (Ab190246). Black - Unlabelled control, cells without incubation with primary antibody.
ZJ25F0826234
危险品化学品经营许可证（带存储）