Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from mouse. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with Flow Cytometry and IF/ICC. No calculations and dilutions are required. Just add an assay dependent volume to stain your cells, and you're ready for the next step in your experiment.
IF analysis of GAPDH (red) in MCF-7 cells. The cells were fixed with 100% methanol for 5 minutes and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with 0.5 μg/mL GAPDH Mouse mAb (Ab155825) in blocking buffer overnight at 4°C. Then incubated with Goat Anti-Mouse IgG H&L (AF594) (Ready to use) (Ab220016) at 2mL/well for 1 hour at room temperature in the dark (red). A 6-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with Recombinant CD45 Antibody (Ab210608). Goat Anti-Mouse IgG H&L (AF594) (Ready to use) (Ab220016) at 100 μL/well was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
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