Recombinant DYKDDDDK Antibody, clone P16A17, could be used for WB, Flow Cytometry, IF/ICC, IP, ELISA and so on.
规格
产品名称
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody
别名
Flag标签抗体 | DYKDDDDK表位标签 | DYKDDDDK抗体
英文别名
DDDDK epitope tag antibody | DDDK antibody | ddk antibody | DYKDDDDK (also known as the FLAG® tag sequence) antibody | DYKDDDDK antibody | DYKDDDDK epitope tag antibody | DYKDDDDK tag antibody | ECS epitope tag antibody | ECS tag antibody | Enterokinase C
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended.
宿主种属
小鼠(Mouse)
特异性
DYKDDDDK epitope tag
种属反应性
不依赖种属(Species independent)
免疫原
Synthetic DYKDDDDK peptide
阳性对照
WB: DYKDDDDK tag transfected HEK293, whole cell lysate Flow Cytometry: HEK293 cells transfected with Flag tag IF/ICC: HEK293 cells transfected with Flag and mCherry tags, HEK293 cells transfected with Flag and eGFP tags IP: HEK293 cells transfected with F
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
储存与运输
储存缓冲液
50% Glycerol, 150 mM NaCl, 10 mM sodium phosphate, pH 7.4
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融.
图片
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab220320) - Western Blot All lanes: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab220320) at 0.5 μg/mL Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 115 kDa Observed band size: 64, 119 kDa Exposure time: 30.0 s
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab220320) - Flow Cytometry Flow Cytometry analysis of HEK293-EGFP-Flag tag cells labelling DYKDDDDK tag with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab220320) (Right panel) compared with Mouse IgG (Ab170221) - Isotype Control (left panel) and detected with Goat Anti-Mouse IgG H&L (AF647) (Ready to use) (Ab218689).
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab220320) - IF IF analysis of DYKDDDDK tag (red) in HEK293-EGFP-Flag Tag cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab220320) at 2.0 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF647) (Ready to use) (Ab218689) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue), and the EGFP protein is green. Images were taken on the confocal laser scanning microscope.
ZJ25F0623115
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