An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells.
Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes.
In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins.
Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance.
The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides.
The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules.
Ubiquitinated by MARCHF1 or MARCHF8 at Lys-244 leading to down-regulation of MHCII. When associated with ubiquitination of the beta chain at 'Lys-254', the down-regulation of MHCII may be highly effective.
宿主种属
小鼠(Mouse)
特异性
Human HLA-DRA
种属反应性
人(Human)
预测反应种属
Gorilla gorilla,Pan paniscus
免疫原
Recombinant protein corresponding to Human HLA-DRA
阳性对照
WB: Raji and Ramos whole cell lysates IF/ICC: Raji cells
偶联
Unconjugated
克隆类型
单克隆抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A purified
浓度
1.0 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, pH 7.2
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。
图片
HLA-DR Mouse mAb (Ab220467) - Western Blot All lanes: HLA-DR Mouse Antibody (Ab220467) at 1/10000 dilution Samples: Lysates at 10 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 29 kDa Observed band size: 32 kDa Exposure time: 30.0 s
HLA-DR Mouse mAb (Ab220467) - IF/ICC analysis of HLA-DR Mouse mAb (Green) in Raji cells. The cells were blocked with 2% BSA for 1 hour at room temperature. Cells were stained with HLA-DR Mouse mAb (Ab220467) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
ZJ25F0622866
危险品化学品经营许可证（带存储）