Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
宿主种属
大鼠(Rat)
特异性
SIGLEC5/CD170
种属反应性
小鼠(Mouse)
阳性对照
Flow: C57BL/6 mouse peritoneal macrophage
偶联
Unconjugated
克隆类型
重组抗体
Format
Whole IgG
轻链亚型
kappa
纯化方法
Protein A/G purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
储存缓冲液
PBS, 0.09% Sodium azide, pH 7.2
防腐剂
0.09% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Two-color flow cytometric analysis of SIGLEC5/CD170 expression on C57BL/6 mouse peritoneal macrophages. C57BL/6 mouse peritoneal macrophages were stained with Recombinant F4/80 Antibody (AF647) (Ab213899) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or Recombinant SIGLEC5/CD170 Antibody (Ab222909; Right panel) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C and detected with SA-PE (rp156150). Two-color flow cytometric dot plots showing the correlated expression of F4/80 versus SIGLEC5/CD170 (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mouse peritoneal macrophages.