ExactAb™,PBS Only,已验证,无内毒素,无动物源,无载体,重组,高性能 级 提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Recombinant DYKDDDDK Antibody, clone P16A17, could be used for WB, Flow Cytometry, IF/ICC, IP, ELISA and so on.
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab224136) - Western Blot
All lanes: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab224136) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 115 kDa
Observed band size: 64, 119 kDa
Exposure time: 30.0 s
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab224136) - Flow Cytometry
Flow Cytometry analysis of HEK293-EGFP-Flag tag cells
labelling DYKDDDDK tag with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab224136) (Right panel) compared with Mouse IgG (Ab170221) - Isotype Control (left panel) and detected with Goat Anti-Mouse IgG H&L (AF647) (Ready to use) (Ab218689).
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab224136) - IF
IF analysis of DYKDDDDK tag (red) in HEK293-EGFP-Flag Tag cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab224136) at 2.0 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF647) (Ready to use) (Ab218689) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue), and the EGFP protein is green. Images were taken on the confocal laser scanning microscope.
1/200
1/200
1/1000 - 1/20000
1/200 – 1/20000
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0623100 |
分析证书 | Ab224136 |
| ZJ25F0623101 |
分析证书 | Ab224136 |
| ZJ25F0623102 |
分析证书 | Ab224136 |
| ZJ25F0623103 |
分析证书 | Ab224136 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 Animal Free 级别指南 → 查看 Carrier Free 级别指南 → 查看 Recombinant 级别指南 → 查看 Validated 级别指南 → 查看 Endotoxin Free 级别指南 → 查看 ExactAb™ 级别指南 → 查看 High Performance 级别指南 → 查看 PBS Only 级别指南 →
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