计算溶液所需的质量、体积或浓度。
EnzymoPure™,重组 级 提供 ≥90%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
甜菜碱-同型半胱氨酸S-甲基转移酶(BHMT)是一种含金属锌的催化酶,它属于转移酶家族,特别是转移那些含一碳基团的甲基,参与甘氨酸,丝氨酸,苏氨酸和蛋氨酸的代谢。现已确定该酶能够和微管蛋白相结合,并链接到自噬体。研究表明该酶能够调节载脂蛋白B (Apolipoprotein B) 的表达,导致相关脂蛋白水平的增加。另外,BHTM是体内重要的Hcy转甲基代谢酶,在调控血浆Hcy浓度的过程中扮演了非常重要的角色。
本公司的BHMT为基因重组产品,无动物源性病毒,产品纯度高,活性好。
BHMT催化的特异性反应如下:
Betaine (甜菜碱) + L-homocysteine (L-同型半胱氨酸) = Dimethylglycine (二甲基甘氨酸) + L-methionine (L-甲硫氨酸)
等电点:10.0
最适pH:8.0
最适温度:37℃
离子: Zn²⁺.
Km(文献报道):甜菜碱0.1mmol/L L-Hcy:0.02mmol/L,不同文献报道各不相同,这里给出为报道相对较多的浓度,实际的Km值需要摸索。
说明: 本品标示量按蛋白质质量计。
Betaine‑homocysteine S‑methyltransferase (BHMT) is a zinc‑containing metalloenzyme that belongs to the transferase family. It specifically transfers methyl groups as one‑carbon units and participates in the metabolism of glycine, serine, threonine and methionine. The enzyme has been identified to bind to tubulin and associate with autophagosomes. Studies have shown that BHMT regulates the expression of apolipoprotein B, leading to elevated levels of related lipoproteins. In addition, BHMT is a key enzyme involved in homocysteine (Hcy) transmethylation in vivo and plays an essential role in regulating plasma Hcy concentration. Our BHMT is a recombinant protein product, free of animal‑derived viruses, with high purity and excellent enzymatic activity. The specific reaction catalyzed by BHMT is as follows: Betaine + L‑homocysteine = Dimethylglycine + L‑methionine
Isoelectric point: 10.0
Optimal pH: 8.0
Optimal temperature: 37 °C
Required ion: Zn²⁺
Kₘ values (literature reports):
Betaine: 0.1 mmol/L
L‑Hcy: 0.02 mmol/L
Reported Kₘ values vary among different publications. The values listed above are the most frequently cited ones. The actual Kₘ should be determined experimentally.
Note: Labeled amount refers to protein mass.