计算溶液所需的质量、体积或浓度。
ActiBioPure™,EnzymoPure™,His标签,生物活性,重组,高性能 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
β-1,4-半乳糖基转移酶 1 (Y285L) 是一种β1,4-N 乙酰半乳糖氨基转移酶,能有效转移 GalNAc 或者 GalNAz 等衍生物到末端 GlcNAc 残基,后者可以应用于抗体-药物偶联物(ADC)作为靶向癌症治疗等。
反应式

产品性质
来源(Source): HEK293
标签(label):C-terminal His Tagged
分子量(Molecular weight): 50.8 kDa
保存缓冲液(storage Buffer): 20mM Tris-HCl, 200 mM NaCl, 20% Glycerin, pH7.4
酶活(Enzyme activity): >1000 U/mg protein
酶活定义(Unit Definition): 在37℃下,每min转移1 nmol GalNAz到GlcNAc所需要的酶量(mg)。
运输和保存方法
冰袋或干冰运输;-20℃保存;有效期 12 个月。
建议使用方法(实际应用可依据实验情况调整)
1. 反应体系: 50 mM Tris-HCl (pH 7.5), 6 mM MnCl₂
2. 反应试剂:
①UDP-GalNAz:抗体(摩尔比)=300:1;
②酶添加量:酶 / 抗体 = 5%;
3. 按照上述条件配制反应液,37℃反应 48 小时;后置于 - 15 至 - 25℃停止反应并进行样品检测。
注意事项
Beta-1,4-galactosyltransferase 1 (Y285L) is a β1,4-N-acetylgalactosaminyltransferase that can efficiently transfer GalNAc or its derivatives such as GalNAz to terminal GlcNAc residues. The latter can be applied to antibody-drug conjugates (ADCs) for targeted cancer therapy and other applications.
Reaction Formula
Product Properties
Source: HEK293
Label: C-terminal His Tagged
Molecular weight: 50.8 kDa
Storage Buffer: 20 mM Tris-HCl, 200 mM NaCl, 20% Glycerin, pH 7.4
Enzyme activity: >1000 U/mg protein
Unit Definition: The amount of enzyme (mg) required to transfer 1 nmol of GalNAz to GlcNAc per minute at 37℃.
Transport and Storage Methods
Transported on ice packs or dry ice; stored at -20℃; valid for 12 months.
Recommended Usage (can be adjusted according to actual experimental conditions)
1. Reaction System: 50 mM Tris-HCl (pH 7.5), 6 mM MnCl₂
2. Reaction Reagents: ① UDP-GalNAz:antibody (molar ratio) = 300:1; ② Enzyme addition amount: enzyme/antibody = 5%
3. Prepare the reaction solution according to the above conditions, incubate at 37℃ for 48 hours; then place at -15 to -25℃ to terminate the reaction and conduct sample detection.
Precautions Recommended Usage
Please pay attention to experimental safety and operate with gloves. This product is for research use only!
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | B1438840 | |
| 分析证书 | B1438840 | |
| 分析证书 | B1438840 |
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