储存缓冲液：Purified Water, 0.05% ProClin 300
阿拉丁黑色乳胶微球由聚苯乙烯乳胶通过特有的深度染色技术染色得到，色彩饱满，染料均匀分布在微球内部，极少吸附在表面或脱落在分散液中。该产品可广泛应用于侧向层析和乳胶凝集试验。
| 产品名称 | 货号 | 表面基团 | 粒径 | 浓度 |
黑色乳胶微球
| B1506732-A200nm
| 羧基（COOH） | 200nm | 4% (40mg/mL) |
黑色乳胶微球
| B1506732-A300nm
| 羧基（COOH）
| 300nm
| 4% (40mg/mL) |
黑色乳胶微球
| B1506732-A400nm
| 羧基（COOH）
| 400nm
| 4% (40mg/mL) |
黑色乳胶微球
| B1506732-B200nm
| 链霉亲和素（SA） | 200nm
| 1% (10mg/mL) |
黑色乳胶微球
| B1506732-B300nm
| 链霉亲和素（SA）
| 300nm
| 1% (10mg/mL) |
黑色乳胶微球
| B1506732-B400nm
| 链霉亲和素（SA）
| 400nm
| 1% (10mg/mL) |
产品特点
特有深度染色技术，颜色深，色彩饱满
仅内部染色，表面清洁，分散体系无染料颗粒
粒径高度均一，CV≤3%，批间差异小
使用说明
抗体偶联方法实例（以表面基团羧基为例）
1、推荐缓冲液
| 名称 | 详情
| 配制方法
|
| MES | 50mM MES, pH 6.0
| 1.06g MES monohydrate (CAS: 145224-94-8) dissolved in 80mL purified water, adjust to pH 6.0 and adjust to 100mL.
|
sulfo-NHS溶液
| 10mg/mL in MES
| 100mg sulfo-NHS (CAS: 145224-94-8) dissolved in 10mL MES buffer.
|
EDC溶液
| 10mg/mL in MES
| 100mg EDC (CAS: 25952-53-8) dissolved in 10mL MES buffer.
|
封闭液
| 50mM HEPES, 1% BSA, pH 8.0
| 1.19g HEPES (CAS: 7365-45-9) dissolved in 80mL purified water, adjust to pH 8.0 and adjust to 100mL. Dissolve 1.0g BSA (CAS: 9048-46-8) in the buffer. |
2、实验步骤
(1) 清洗：取 120 μL 的黑色乳胶微球（4% 固含量），用超声波细胞粉碎机超声分散，离心（离心力＞15000）5 分钟，移除上清， 用 MES 离心洗涤 1 次。
(2) 活化：加入 300 μL 的 MES，用碎冰预冷后，加入 40 μL 的 sulfo-NHS 溶液，摇匀，再加入 20 μL 的 EDC 溶液，摇匀后室温反应 20 分钟。离心（离心力＞15000）5 分钟，移除上清，用 MES 离心洗涤 1 次。
(3) 抗体偶联：加入 480 μL 的 MES，超声分散，加入 160 μg 的抗体，摇匀，室温反应 4 小时。离心（离心力＞15000）5 分钟，移除上清。
(4) 封闭：加入 480 μL 的封闭液，超声分散，室温反应 1 小时。离心（离心力＞15000）5 分钟，移除上清，用封闭液离心洗涤 1 次。
(5) 保存：加入 480 μL 的保存液（与封闭液相同，可添加少量稳定剂），超声分散，2~8℃保存。
Storage buffer: Purified Water, 0.05% ProClin 300
Aladdin Black Latex Beads are produced through a proprietary deep-dyeing technique applied to polystyrene latex, resulting in vibrant color saturation. The dye is uniformly distributed within the microspheres, with minimal adsorption on the surface or release into the dispersion medium. This product is widely applicable in lateral flow assays and latex agglutination tests.
Product Name
| Catalog Number
| Surface Group
| Particle Size
| Concentration
|
Black Latex Beads
| B1506732-A200nm
| Carboxyl (COOH)
| 200nm | 4% (40mg/mL) |
Black Latex Beads
| B1506732-A300nm
| Carboxyl (COOH)
| 300nm
| 4% (40mg/mL) |
Black Latex Beads
| B1506732-A400nm
| Carboxyl (COOH)
| 400nm
| 4% (40mg/mL) |
Black Latex Beads
| B1506732-B200nm
| Streptavidin (SA)
| 200nm
| 1% (10mg/mL) |
Black Latex Beads
| B1506732-B300nm
| Streptavidin (SA)
| 300nm
| 1% (10mg/mL) |
Black Latex Beads
| B1506732-B400nm
| Streptavidin (SA)
| 400nm
| 1% (10mg/mL) |
Product Features
Proprietary deep-dyeing technology ensures deep, vibrant colors.
Dye is incorporated internally, resulting in a clean surface and a dispersion medium free of dye particles.
Highly uniform particle size with CV ≤ 3% and low batch-to-batch variation.
Instructions for Use
Example Antibody Conjugation Protocol (Using Carboxyl-functionalized Microspheres)
1. Recommended Buffers
Name
| Details
| Preparation Method
|
MES Buffer
| 50mM MES, pH 6.0
| 1.06g MES monohydrate (CAS: 145224-94-8) dissolved in 80mL purified water, adjust to pH 6.0 and adjust to 100mL.
|
sulfo-NHS Solution
| 10mg/mL in MES
| 100mg sulfo-NHS (CAS: 145224-94-8) dissolved in 10mL MES buffer.
|
EDC Solution
| 10mg/mL in MES
| 100mg EDC (CAS: 25952-53-8) dissolved in 10mL MES buffer.
|
Blocking Buffer
| 50mM HEPES, 1% BSA, pH 8.0
| 1.19g HEPES (CAS: 7365-45-9) dissolved in 80mL purified water, adjust to pH 8.0 and adjust to 100mL. Dissolve 1.0g BSA (CAS: 9048-46-8) in the buffer. |
2. Experimental Procedure
(1) Washing: Take 120 μL of Black Latex Beads (4% solid content). Disperse by ultrasonication using an ultrasonic cell disruptor. Centrifuge (RCF > 15,000 × g) for 5 minutes and remove the supernatant. Wash once by centrifugation with MES Buffer.
(2) Activation: Add 300 μL of MES Buffer, pre-cooled on crushed ice. Add 40 μL of sulfo-NHS Solution, mix well. Then add 20 μL of EDC Solution, mix well, and react at room temperature for 20 minutes. Centrifuge (RCF > 15,000 × g) for 5 minutes, remove the supernatant, and wash once by centrifugation with MES Buffer.
(3) Antibody Conjugation: Add 480 μL of MES Buffer, disperse by ultrasonication. Add 160 μg of antibody, mix well, and react at room temperature for 4 hours. Centrifuge (RCF > 15,000 × g) for 5 minutes and remove the supernatant.
(4) Blocking: Add 480 μL of Blocking Buffer, disperse by ultrasonication, and react at room temperature for 1 hour. Centrifuge (RCF > 15,000 × g) for 5 minutes, remove the supernatant, and wash once by centrifugation with Blocking Buffer.
(5) Storage: Add 480 μL of Storage Buffer (same as Blocking Buffer; small amounts of stabilizers may be added), disperse by ultrasonication, and store at 2–8 °C.
危险品化学品经营许可证（带存储）