甜菜碱(Betaine)是一种碱性物质,CAS号为107-43-7,分子量为117.15。在PCR反应中,甜菜碱能够有效防止Taq DNA聚合酶在高温变性过程中活性降低。
甜菜碱溶液 (5 mol/L, PCR级) 专为PCR反应优化,其主要功能包括:
适用于富含GC模板的PCR扩增;
降低变性温度对碱基组成的依赖性;
提高Taq DNA聚合酶的稳定性。
操作步骤
将甜菜碱溶液 (5 mol/L, PCR级) 加入PCR反应体系,其工作浓度一般为1~2 mol/L。
注意事项
1. 如遇环境温度较低,溶液易产生沉淀,可以温浴加热促溶。
2. 本产品仅用于科研实验,不可直接用于人体或临床诊断。
3. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
Betaine is an alkaline substance with a CAS number of 107-43-7 and a molecular weight of 117.15. In PCR reactions, betaine effectively prevents the loss of Taq DNA polymerase activity during high-temperature denaturation.
Betaine Solution (5 mol/L, PCR Grade) is specifically optimized for PCR reactions. Its main functions include:
Suitable for PCR amplification of GC-rich templates.
Reduces the dependence of denaturation temperature on base composition.
Enhances the stability of Taq DNA polymerase.
Procedure
Add Betaine Solution (5 mol/L, PCR Grade) to the PCR reaction system. Its working concentration is typically 1–2 mol/L.
Precautions
If precipitation occurs due to low ambient temperature, the solution can be heated in a water bath to dissolve.
This product is for research use only and is not intended for direct human application or clinical diagnosis.
For your safety and health, please wear a lab coat and disposable gloves during operation.