Name: 血液基因组DNA提取试剂盒
SKU: B1522828
Availability: InStock

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

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为什么选择此级别

for DNA and RNA applications,分子生物学级,生物活性级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

室温。常规运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

本试剂盒适合从血液中快速简单地提取基因组DNA，提取的基因组DNA片段大，纯度高，稳定性好，可直接用于PCR、酶切和杂交等实验。提取过程无需酚氯仿抽提，血液经裂解液裂解和蛋白酶K消化，然后在无水乙醇的调节下可与吸附柱WHF结合，经过快速充分的洗涤去除残留的蛋白质和盐分等杂质，最后DNA溶解于Buffer EB中。

产品号	组件	物理外观	50T	储存
B1522828A	Buffer FL	液体	60 mL	RT.
B1522828B	Buffer DP	液体	15 mL	RT.
B1522828C	Buffer DBL	液体	11 mL	RT.
B1522828D	Buffer DBT	液体	20 mL	RT.
B1522828E	Buffer PW1	液体	17 mL	RT.
B1522828F	Buffer PW3	液体	15 mL	RT.
B1522828G	Buffer EB	液体	15 mL	RT.
B1522828H	蛋白酶K	液体	1.2 mL	RT.
B1522828I	吸附柱WHF (含2mL收集管)	—	1 EA×50	RT.

本试剂盒所有组分可置于室温干燥条件下保存15个月。

操作步骤

使用前请先在Buffer PW1中加入22 mL无水乙醇，Buffer PW3中加入60 mL的无水乙醇。

1. 样本处理

1.1 哺乳动物血液

取200 μL新鲜或抗凝处理的血液，直接按照后续步骤2-9进行操作；若血液样品体积不足200 μL，需用Buffer DP补足至200 μL。

【注意】若需处理的血液体积超过200 μL，需先在血液样品中加入1-2.5倍体积的Buffer FL，颠倒混匀后，12,000 rpm（13,680 × g）离心1 min，弃去上清液；若裂解不彻底，可再次加入1-2.5倍血液体积的Buffer FL重复裂解一次。裂解完成后，向沉淀的细胞核中加入200 μL Buffer DP，振荡至彻底混匀。

1.2 禽类动物血液

取5-20 μL新鲜或抗凝处理的血液，用Buffer DP补足至200 μL。

1.3 血凝块

(1) 将血凝块转移至液化柱GLF（需用户自备），12,000 rpm（13,680 × g）离心1 min，收集滤液；若处理的血凝块体积较大，可分多次过柱并合并收集滤液。

(2) 取100 μL-1 mL收集到的滤液，加入1-2.5倍体积的Buffer FL，颠倒混匀后，12,000 rpm（13,680 × g）离心1 min，弃去上清液；若裂解不彻底，可再次加入1-2.5倍血液样品体积的Buffer FL重复裂解一次。裂解完成后，向沉淀的细胞核中加入200 μL Buffer DP，振荡至彻底混匀。

【注意】若需去除提取的DNA中的RNA，可在样本处理完成后，加入4 μL RNase A（100 mg/mL）溶液（客户自备），涡旋振荡混匀15 sec，室温放置5-10 min即可。

2. 向处理好的200 μL样本中，加入20 μL 蛋白酶K和200 μL Buffer DBL，充分颠倒混匀，56℃孵育10 min，溶液应变为清亮；若溶液未彻底变清亮，请延长孵育时间至溶液完全清亮。

【注意】加入Buffer DBL时可能会产生白色沉淀，通常在56℃孵育过程中会自行消失，不会影响后续实验。若血液样品已提前经Buffer FL处理，大多数情况下，加入Buffer DBL充分颠倒混匀后，室温放置5 min（无需加热，期间颠倒混匀1-2次），即可获得高质量的基因组DNA。

3. 加入350 μL Buffer DBT，充分颠倒混匀，此时溶液中可能会出现絮状沉淀，属于正常现象，无需处理。

4. 将步骤3中得到的溶液和絮状沉淀一起转入吸附柱WHF（将吸附柱放入配套的2 mL收集管中），12,000 rpm（13,680 × g）离心30 sec，弃去收集管中的废液，将吸附柱重新放回收集管中。

5. 向吸附柱中加入700 μL Buffer PW1（使用前已加入无水乙醇），12,000 rpm（13,680 × g）离心30 sec，弃去废液，将吸附柱放回收集管中。

6. 向吸附柱中加入700 μL Buffer PW3（使用前已加入无水乙醇），12,000 rpm（13,680 × g）离心30 sec，弃去废液，将吸附柱放回收集管中。

7. 重复步骤6的操作，进一步去除吸附柱上残留的杂质。

8. 将吸附柱连同收集管一起，12,000 rpm（13,680 × g）离心2 min，彻底去除吸附柱中残留的洗涤液（避免残留乙醇影响后续DNA洗脱和实验）。

9. DNA洗脱

将吸附柱从收集管中取出，转入一个新的1.5 mL离心管中，开盖放置2 min，彻底挥发吸附柱中残留的乙醇。向吸附膜中间部位悬空滴加50-200 μL Buffer EB，室温放置1 min，12,000 rpm（13,680 × g）离心30 sec，收集离心管中的DNA溶液，可立即用于后续实验，或置于-20℃长期保存。

【注意】

(1) 若下游实验对pH值或EDTA敏感，可改用灭菌水进行洗脱。洗脱液的pH值对洗脱效率影响较大，若用水作为洗脱液，需保证其pH值在7.0-8.5之间（可使用NaOH调节），pH值低于7.0会导致洗脱效率下降。

(2) 若需提高DNA的终浓度，可将步骤9收集到的DNA洗脱液重新加至同一吸附膜上，室温放置2 min，12,000 rpm（13,680 × g）离心30 sec，再次收集洗脱液即可。

(3) 若需长期保存DNA，建议使用Buffer EB进行洗脱，并将DNA溶液置于-20℃保存，可有效防止DNA降解。

DNA浓度及纯度检测

提取的基因组DNA片段大小，受样品保存时间、操作过程中的剪切力等因素影响。纯化后的DNA片段可通过琼脂糖凝胶电泳和紫外分光光度计两种方法，检测其浓度与纯度。

1. 浓度检测

通过紫外分光光度计测量样品在260 nm处的吸光度（A₂₆₀），确定DNA浓度。若需稀释样品，建议使用Buffer EB进行稀释，若用水稀释，可能导致检测数值不准确。260 nm处的吸光度应控制在0.1-1.0范围内，以保证检测结果的准确性。换算标准：260 nm处1个单位的吸光度，相当于50 µg/mL双链DNA，或40 µg/mL单链DNA。

2. 纯度检测

通过检测A₂₆₀/A₂₈₀的比值判断DNA纯度，合格的基因组DNA，其A₂₆₀/A₂₈₀比值应在1.7-1.9之间。若洗脱时未使用Buffer EB，而是使用ddH₂O，可能会导致比值偏低，这是由于pH值和离子存在影响光吸收值所致，并不代表DNA纯度不足。

This kit is suitable for rapid and simple extraction of genomic DNA from blood. The extracted genomic DNA has large fragments, high purity, and good stability, and can be directly used in experiments such as PCR, restriction enzyme digestion, and hybridization. The extraction process does not require phenol‑chloroform extraction. The blood is lysed with a lysis buffer and digested with proteinase K, then in the presence of absolute ethanol the DNA binds to the WHF spin column. After thorough washing to remove residual proteins, salts, and other impurities, the DNA is finally eluted with Buffer EB.

Product No.	Component	Appearance	50T	Storage
B1522828A	Buffer FL	Liquid	60 mL	RT
B1522828B	Buffer DP	Liquid	15 mL	RT
B1522828C	Buffer DBL	Liquid	11 mL	RT
B1522828D	Buffer DBT	Liquid	20 mL	RT
B1522828E	Buffer PW1	Liquid	17 mL	RT
B1522828F	Buffer PW3	Liquid	15 mL	RT
B1522828G	Buffer EB	Liquid	15 mL	RT
B1522828H	Proteinase K	Liquid	1.2 mL	RT
B1522828I	WHF Spin Column (with 2 mL collection tube)	—	1 EA × 50	RT

All components of this kit can be stored under dry conditions at room temperature for 15 months.

Procedure

Before use, add 22 mL of absolute ethanol to Buffer PW1 and 60 mL of absolute ethanol to Buffer PW3.

1. Sample Processing

1.1 Mammalian blood

Take 200 μL of fresh or anticoagulant‑treated blood and proceed directly to steps 2–9. If the blood sample volume is less than 200 μL, make up to 200 μL with Buffer DP.

【Note】 If the blood volume to be processed exceeds 200 μL, first add 1–2.5 volumes of Buffer FL to the blood sample, mix by inversion, centrifuge at 12,000 rpm (13,680 × g) for 1 min, and discard the supernatant. If lysis is incomplete, repeat the lysis by adding 1–2.5 volumes of Buffer FL (relative to the original blood volume). After lysis, add 200 μL of Buffer DP to the pelleted nuclei and vortex until thoroughly mixed.

1.2 Avian blood

Take 5–20 μL of fresh or anticoagulant‑treated blood and make up to 200 μL with Buffer DP.

1.3 Blood clots

(1) Transfer the blood clot to a GLF liquefaction column (user‑supplied). Centrifuge at 12,000 rpm (13,680 × g) for 1 min and collect the filtrate. If the clot volume is large, pass through the column multiple times and combine the filtrates.

(2) Take 100 μL–1 mL of the collected filtrate, add 1–2.5 volumes of Buffer FL, mix by inversion, centrifuge at 12,000 rpm (13,680 × g) for 1 min, and discard the supernatant. If lysis is incomplete, repeat the lysis by adding 1–2.5 volumes of Buffer FL (relative to the sample volume). After lysis, add 200 μL of Buffer DP to the pelleted nuclei and vortex until thoroughly mixed.

【Note】 If removal of RNA from the extracted DNA is desired, after sample processing add 4 μL of RNase A solution (100 mg/mL) (user‑supplied), vortex for 15 sec, and let stand at room temperature for 5–10 min.

2. To the processed 200 μL sample, add 20 μL of Proteinase K and 200 μL of Buffer DBL. Mix thoroughly by inversion and incubate at 56°C for 10 min. The solution should become clear. If it does not become completely clear, extend the incubation time until fully clear.

【Note】 White precipitate may form upon addition of Buffer DBL, but it usually disappears during incubation at 56°C and does not affect subsequent steps. If the blood sample has been pre‑treated with Buffer FL, in most cases, after adding Buffer DBL and mixing thoroughly by inversion, let stand at room temperature for 5 min (no heating; invert to mix 1–2 times during this period) to obtain high‑quality genomic DNA.

3. Add 350 μL of Buffer DBT and mix thoroughly by inversion. Flocculent precipitate may appear in the solution; this is normal and requires no further treatment.

4. Transfer the solution and the flocculent precipitate from step 3 into a WHF spin column (placed in the provided 2 mL collection tube). Centrifuge at 12,000 rpm (13,680 × g) for 30 sec. Discard the flow‑through and place the spin column back into the collection tube.

5. Add 700 μL of Buffer PW1 (with ethanol pre‑added) to the spin column. Centrifuge at 12,000 rpm (13,680 × g) for 30 sec. Discard the flow‑through and return the spin column to the collection tube.

6. Add 700 μL of Buffer PW3 (with ethanol pre‑added) to the spin column. Centrifuge at 12,000 rpm (13,680 × g) for 30 sec. Discard the flow‑through and return the spin column to the collection tube.

7. Repeat step 6 to further remove residual impurities on the spin column.

8. Centrifuge the spin column together with the collection tube at 12,000 rpm (13,680 × g) for 2 min to completely remove residual wash buffer (this avoids residual ethanol affecting subsequent DNA elution and experiments).

9. DNA elution

Transfer the spin column to a new 1.5 mL centrifuge tube. Open the cap and let stand for 2 min to allow residual ethanol to evaporate completely. Add 50–200 μL of Buffer EB dropwise to the center of the membrane without touching it. Let stand at room temperature for 1 min. Centrifuge at 12,000 rpm (13,680 × g) for 30 sec. Collect the DNA solution in the centrifuge tube. The DNA can be used immediately for downstream experiments or stored at –20°C for long‑term preservation.

【Notes】

(1) If downstream applications are sensitive to pH or EDTA, sterile water can be used for elution instead. The pH of the elution buffer significantly affects elution efficiency. If water is used, ensure its pH is between 7.0 and 8.5 (adjust with NaOH). pH values below 7.0 will reduce elution efficiency.

(2) To increase the final DNA concentration, reload the collected DNA eluate from step 9 onto the same membrane, let stand at room temperature for 2 min, centrifuge at 12,000 rpm (13,680 × g) for 30 sec, and collect the eluate again.

(3) For long‑term DNA storage, it is recommended to elute with Buffer EB and store the DNA solution at –20°C to effectively prevent DNA degradation.

DNA Concentration and Purity Assessment

The fragment size of the extracted genomic DNA is influenced by factors such as sample storage time and shear forces during handling. The purified DNA can be assessed for concentration and purity by agarose gel electrophoresis and UV spectrophotometry.

1. Concentration determination

Measure the absorbance of the sample at 260 nm (A260) using a UV spectrophotometer to determine DNA concentration. If dilution is required, Buffer EB is recommended. Dilution with water may lead to inaccurate readings. The absorbance at 260 nm should be kept within 0.1–1.0 to ensure accuracy. Conversion: 1 unit of absorbance at 260 nm corresponds to 50 µg/mL of double‑stranded DNA or 40 µg/mL of single‑stranded DNA.

2. Purity assessment

Evaluate DNA purity by the A260/A280 ratio. For high‑quality genomic DNA, the A260/A280 ratio should be between 1.7 and 1.9. If ddH₂O instead of Buffer EB is used for elution, the ratio may be lower due to the influence of pH and ions on light absorption; this does not necessarily indicate low DNA purity.

规格

别名

血液DNA提取试剂盒 | 柱式血液基因组 DNA 抽提试剂盒

英文别名

Blood Genomic DNA Extraction Kit (Spin Column) | Blood DNA Kit

规格或纯度

生物活性,for DNA and RNA applications,分子生物学级

英文名称

Blood DNA Kit

应用

DNA Extraction, 核酸提取与纯化

储存与运输

储存条件

室温

运输条件

常规运输

稳定性与储存

Store at room temperature long term (15 months).

组分与储存

产品号	组件	物理外观	50T	储存
B1522828A	Buffer FL	液体	60 mL	RT
B1522828B	Buffer DP	液体	15 mL	RT
B1522828C	Buffer DBL	液体	11 mL	RT
B1522828D	Buffer DBT	液体	20 mL	RT
B1522828E	Buffer PW1	液体	17 mL	RT
B1522828F	Buffer PW3	液体	15 mL	RT
B1522828G	Buffer EB	液体	15 mL	RT
B1522828H	蛋白酶K	液体	1.2 mL	RT
B1522828I	吸附柱WHF (含2mL收集管)	—	1 EA × 50	RT

技术文档

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高级数据

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批号(Lot Number)	证书类型	货号
ZJ26F0636844	分析证书	B1522828

技术文档和文章

b1522828 血液基因组dna提取试剂盒说明书.pdf

b1522828 blood dna kit manual.pdf

DNA提取方法比较：酚氯仿法、硅胶柱法、磁珠法、CTAB法与快速裂解法

目录：技术文章

探索主题:

DNA提取磁珠法基因组DNA 酚氯仿抽提硅胶柱法 CTAB法 DNA纯化核酸质量评价

Comparison of DNA Extraction Methods: Phenol-Chloroform Extraction, Silica Column Method, Magnetic Bead Method, CTAB Method, and Rapid Lysis Method

目录：技术文章

探索主题:

DNA extraction Genomic DNA Phenol-chloroform extraction Silica column method Magnetic bead method CTAB method DNA purification Nucleic acid quality assessment

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

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血液基因组DNA提取试剂盒

储存与运输

质量文档

文献证明

概述

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

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