计算溶液所需的质量、体积或浓度。
BioReagent,冻干粉,天然 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
纤维蛋白原是一种高丰度血浆蛋白(5–10 μM),在肝脏中合成。完整蛋白分子量为 330 kDa,由 3 对通过二硫键连接的多肽链构成,分别命名为 Aα、Bβ 和 γ 链。纤维蛋白原为三球状蛋白,包含一个中心 E 结构域与两端 D 结构域。 经凝血酶水解后,先释放纤维蛋白肽 A(FPA,Aα1–16),随后释放纤维蛋白肽 B(FPB,Bβ1–14);生成的纤维蛋白单体以半重叠方式聚合,形成不溶性纤维蛋白原纤维。由于 FPA 与 FPB 被切除,生成的纤维蛋白链称为 α、β、γ 链。聚合后的纤维蛋白在活化的凝血因子 XIII(转谷氨酰胺酶)作用下进一步稳定,该酶可在纤维蛋白分子 γ 链间形成酰胺键,在 α 链间也可形成酰胺键(作用程度较弱)。
纤溶酶对纤维蛋白原水解时,首先切除 Aα 链 C 端残基,生成 X 片段(完整 D-E-D 结构,仍具备可凝固性)。
牛纤维蛋白原,去除纤溶酶原由新鲜采集的牛血浆纯化制备,经赖氨酸琼脂糖层析柱去除纤溶酶原。
全部牛源原料取自月龄≤30 个月的美国牛只。
Fibrinogen is an abundant plasma protein (5-10 uM) synthesized in the liver. The intact protein has a molecular weight of 330 kDa and is composed of 3 pairs of disulphide-bound polypeptide chains named Aα, Bβ and γ. Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains. Proteolysis by Thrombin results in release of Fibrinopeptide A (FPA, Aα1-16) followed by Fibrinopeptide B (FPB, Bβ1-14) and the Fibrin Monomers that result polymerize in a half-overlap fashion to form insoluble Fibrin fibrils. The chains of Fibrin are referred to as α, β and γ, due to the removal of FPA and FPB. The polymerised Fibrin is subsequently stabilized by the transglutaminase activated Factor XIII that forms amide linkages between γ chains and, to a lesser extent, α chains of the Fibrin molecules. Proteolysis of Fibrinogen by Plasmin initially liberates C-terminal residues from the Aα chain to produce Fragment X (intact D-E-D, which is still clottable).
The bovine Fibrinogen - Plasminogen depleted, was purified from freshly collected bovine plasma. The plasminogen was depleted using a lysine-sepharose column.
All bovine material isfrom U.S. cows ≤ 30 months of age.
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