计算溶液所需的质量、体积或浓度。
胆汁盐是一种有机钠盐,包含任何胆汁酸与甘氨酸或牛磺酸的偶联物。胆汁盐由四种不同的胆汁酸组成:胆酸、脱氧胆酸、鹅去氧胆酸和石胆酸。
胆汁盐是在受控条件下由纯化的新鲜胆汁提取出来,可用于细菌培养基,在细胞培养基中它作为一种选择性抑制剂,可以分离和鉴定病原体。例如,麦康凯琼脂/肉汤。
应用
在进行的实验中,胆汁盐可以用作选择性试剂之一,以防止受损的大肠杆菌细胞的生长,以鉴定选择性培养基中的特异性试剂,其将抑制受损的大肠杆菌ML30 细胞的生长。它也可以用于创伤弧菌(贝内克氏菌属)的分离鉴定,它是一种嗜盐菌,可导致几种人类传染病。
Bile salt is an organic sodium salt, which contains any conjugate of Bile acid with glycine or Taurine. Bile salts are composed of four different Bile acid: cholic acid, deoxycholic acid, Chenodeoxycholic acid and Lithocholic acid.
Bile salt is extracted from purified fresh bile under controlled conditions and can be used in bacterial culture medium. As a selective inhibitor in cell culture medium, it can isolate and identify pathogens. For example, MacConkey agar/broth.
Description
Bile salts may be used as one of the selective agents to prevent the growth of injuredEscherichia colicells, in an experiment done in order to identify the specific agent in the selective media which would inhibit the growth of the injuredEscherichia coliML30 cells. It may also be used for identification of isolates ofVibrio(Beneckea)vulnificuswhich is a halophilic bacterium, found responsible for causing several human infections.