计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥95% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Br-DAPI is a marker dye in DAPI series. DAPI is a fluorescent dye that binds strongly to DNA. It binds to the AT base pair of the double-stranded DNA minor groove, and one DAPI molecule can occupy three base pair positions. The fluorescence intensity of DAPI molecules bound to double-stranded DNA is increased by about 20 times, and it is commonly observed with fluorescence microscopy, and the amount of DNA can be determined based on the intensity of fluorescence. In addition, because DAPI can pass through intact cell membranes, it can be used to stain both live and fixed cells. Storage: Keep away from light.
In Vitro
General Protocol 1. Preparation of DAPI working solution 1.1 Preparation of the stock solution Dissolve 1 mg DAPI in 1 mL ddH 2 O to obtain 1 mg/mL of stock solution. Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of DAPI working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 ug/mL of working solution. Note: Please adjust the concentration of DAPI working solution according to the actual situation. 2. Cell staining 2.1 Suspension cells（6-well plate） a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10 6 /mL. b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes. c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant. d. Wash twice with PBS, 5 minutes each time. e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes. d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry. Storage -20°C, 1 year. Protect from light Precautions 1. Please adjust the concentration of DAPI working solution according to the actual situation. 2. This product is for R&D use only, not for drug, household, or other uses. 3. For your safety and health, please wear a lab coat and disposable gloves to operate. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Form:Solid
规格
别名
溴一DAPI
规格或纯度
≥95%
英文名称
Br-DAPI
生化机理
Br-DAPI 是 DAPI 系列中的一种标记染料。DAPI 是一种能与 DNA 强结合的荧光染料。它与双链 DNA 小沟的 AT 碱基对结合，一个 DAPI 分子可占据三个碱基对位置。D
储存条件
避光,-80℃储存
运输条件
特低温运输
纯度
≥95%
产品线
生物产品
名称和识别符
分子类型
小分子
分子量
356.22
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）