Name: CWE9600血液DNA提取试剂盒
SKU: B669919
Availability: InStock

概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 技术文档和文章溶液计算器

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为什么选择此级别

，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

室温。常规运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

B669919	Component	96 T	Storage
B669919A	96 DW Plate	6 Plates	RT
B669919B	Spin Tips Pack	1 Pack	RT
B669919C	Buffer ML	24 mL	RT
B669919D	Buffer KL	80 mL	RT
B669919E	Buffer CW1 (concentrate)	60 mL	RT
B669919F	Buffer GW2 (concentrate)	26 mL	RT
B669919G	Buffer MW3	80 mL	RT
B669919H	Buffer EB	30 mL	RT
B669919I	Proteinase K (Dry powder)	2×25 mg	RT
B669919J	Proteinase K Storage Buffer	2×1.25 mL	RT
B669919K	Magbeads PN	1 mL	RT

产品简介

该试剂盒提供了一种与CWE9600匹配，从300 μl血液中提取DNA的方案。试剂按要求加入96 DW深孔板后，按相应顺序将其放入CWE9600中，约50分钟即可得到高质量的DNA。提取得到的DNA稳定、可靠，可用于PCR 、芯片检测以及高通量测序等下游实验。

自备仪器、试剂

1 ．全自动核酸提取仪CWE9600

2 ．异丙醇

3 ．无水乙醇

实验前准备及重要注意事项

1 ．向25 mg Proteinase K干粉中加入1.25 ml Proteinase K Storage Buffer使其完全溶解后于 -20℃保存。配置好的Proteinase K溶液勿长时间室温放置，避免反复冻融，以免影响其活性。

2 ．向装有Magbeads PN的试剂瓶中加入33 ml异丙醇，混匀后备用。

3 ．第一次使用前按照试剂瓶标签向Buffer CW1和Buffer GW2中加入无水乙醇并做好标记。

操作步骤

1 ．按下表向96 DW 深孔板中加入相应试剂。

Position	Reagent
Plate 1	ProteinaseK: 20μl Sample: 300μl Buffer ML: 200μl
Plate 2	Buffer KL: 750μl
Plate 3	Buffer CW1:750μl
Plate 4	Buffer GW2:750μl
Plate 5	Buffer MW3:750μl
Plate 6	Buffer EB: 100μl

2．将加入试剂的深孔板及Spin tips pack放入核酸提取仪相应位置。

3 ．运行Cowin Blood 300程序，约23分钟后仪器暂停。将Plate 1 从仪器中取出，每孔加入310 ul充分混匀的异丙醇与磁珠混合物。

4 ．将Plate 1 放回仪器中，继续运行程序。约35分钟后程序运行结束，取出磁套和深孔板。

5 ．将Plate 6中的洗脱产物转移至1.5 ml离心管中低温保存。

B669919	Component	96 T	Storage
B669919A	96 DW Plate	6 Plates	RT
B669919B	Spin Tips Pack	1 Pack	RT
B669919C	Buffer ML	24 mL	RT
B669919D	Buffer KL	80 mL	RT
B669919E	Buffer CW1 (concentrate)	60 mL	RT
B669919F	Buffer GW2 (concentrate)	26 mL	RT
B669919G	Buffer MW3	80 mL	RT
B669919H	Buffer EB	30 mL	RT
B669919I	Proteinase K (Dry powder)	2×25 mg	RT
B669919J	Proteinase K Storage Buffer	2×1.25 mL	RT
B669919K	Magbeads PN	1 mL	RT

Product Introduction
This reagent kit provides a match with CWE9600, ranging from 300 μ A plan for extracting DNA from blood. After adding the reagents to the 96 DW deep well plate as required, they are placed in the corresponding order in CWE9600, and high-quality DNA can be obtained in about 50 minutes. The extracted DNA is stable and reliable, and can be used for downstream experiments such as PCR, chip detection, and high-throughput sequencing.

Self provided instruments and reagents
1. Fully automatic nucleic acid extractor CWE9600
2. Isopropanol
3. Anhydrous ethanol
Preparation and important precautions before the experiment
1. Add 1.25 ml of Protein K Storage Buffer to 25 mg of Protein K dry powder until completely dissolved, and store at -20 ℃. The prepared Protein K solution should not be left at room temperature for a long time to avoid repeated freeze-thaw cycles, which may affect its activity.
2. Add 33 ml of isopropanol (recommended to use isopropanol from Beijing Chemical Factory) to the reagent bottle containing Magheads PN, mix well, and set aside.
3. Before the first use, add anhydrous ethanol to Buffer CW1 and Buffer GW2 according to the label of the reagent bottle and mark them properly.
Operation steps
1. Add the corresponding reagents to the 96 DW deep well plate according to the table below.

Position	Reagent
Plate 1	ProteinaseK: 20μl Sample: 300μl Buffer ML: 200μl
Plate 2	Buffer KL: 750μl
Plate 3	Buffer CW1:750μl
Plate 4	Buffer GW2:750μl
Plate 5	Buffer MW3:750μl
Plate 6	Buffer EB: 100μl

2. Place the deep well plate and Spin tips pack containing the reagent into the corresponding position of the nucleic acid extractor.
3. Run the Cowin Blood 300 program and the instrument will pause after approximately 23 minutes. Remove Plate 1 from the instrument and add 310 ul of well mixed isopropanol and magnetic bead mixture to each well.
4. Put Plate 1 back into the instrument and continue running the program. After about 35 minutes, the program ends and the magnetic sleeve and deep hole plate are removed.
5. Transfer the elution products from Plate 6 to a 1.5 ml centrifuge tube for low-temperature storage.

规格

英文名称

CWE9600 Blood DNA Kit

储存与运输

储存条件

室温

运输条件

常规运输

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

b669919_cwe9600 blood dna kit.pdf

DNA提取方法比较：酚氯仿法、硅胶柱法、磁珠法、CTAB法与快速裂解法

目录：技术文章

探索主题:

DNA提取磁珠法基因组DNA 酚氯仿抽提硅胶柱法 CTAB法 DNA纯化核酸质量评价

Comparison of DNA Extraction Methods: Phenol-Chloroform Extraction, Silica Column Method, Magnetic Bead Method, CTAB Method, and Rapid Lysis Method

目录：技术文章

探索主题:

DNA extraction Genomic DNA Phenol-chloroform extraction Silica column method Magnetic bead method CTAB method DNA purification Nucleic acid quality assessment

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

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