计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
| 产品介绍： |
| Western Blot 实验中，为防止印迹膜与检测系统中的蛋白成分（如抗体IgG等）发生非特异性吸附，导致实验结果不清晰，通常在进行一抗孵育前需预先对印迹膜上的非特异结合位点进行封闭。本产品以BSA为基础，配合其他有效成份，可快速高效地封闭膜上多余的结合部位，减少非特异结合情况的产生，降低背景，适用于各种转印膜的封闭，操作方便快捷。另外，本产品特别添加了蛋白稳定剂，可保证每次实验封闭效果稳定可靠。 |
| 注意事项： |
| 1．每次使用时，必须保证封闭液将印迹膜完全浸没，以免影响封闭效果。 2．本产品含有防腐剂，操作时请佩戴手套进行防护。 3．本产品仅用于科研，不能用于人体实验或人体治疗。 |
| 使用说明： |
| 1．本产品无需稀释，开盖即用。 2．目的蛋白转移完毕后，将印迹膜放入容器中，加入适量BSA Blocking Buffer（以完全覆盖印迹膜为宜）。 3．室温孵育30分钟至2小时，必要时可2-8℃孵育过夜。 4．封闭结束后弃去BSA Blocking Buffer，进行Western Blot后续操作。 |
| Product Introduction: |
| In Western Blot experiments, in order to prevent non-specific adsorption between the imprinting membrane and protein components in the detection system (such as antibodies IgG, etc.), which may lead to unclear experimental results, it is usually necessary to block the non-specific binding sites on the imprinting membrane before incubating with primary antibodies. This product is based on BSA and combined with other active ingredients to quickly and efficiently seal excess binding sites on the membrane, reducing the occurrence of non-specific binding and lowering the background. It is suitable for sealing various transfer films and is easy and fast to operate. In addition, this product has specially added protein stabilizers to ensure stable and reliable sealing effects in each experiment. |
| Precautions： |
| 1. During each use, it is necessary to ensure that the sealing solution completely submerges the imprinting film to avoid affecting the sealing effect. 2. This product contains preservatives, please wear gloves for protection during operation. 3. This product is only used for scientific research and cannot be used for human experiments or treatments. |
| Instructions for Use： |
| 1. This product does not require dilution and can be used immediately after opening the lid. 2. After the transfer of the target protein is completed, place the imprinting membrane into a container and add an appropriate amount of BSA Blocking Buffer (preferably to completely cover the imprinting membrane). 3. Incubate at room temperature for 30 minutes to 2 hours, and if necessary, incubate overnight at 2-8 ℃. 4. After the closure is completed, discard the BSA Blocking Buffer and perform subsequent Western Blot operations. |
规格
别名
WB封闭液（含BSA） | Western blot封闭液(含BSA) | BSA封闭液
英文别名
Blocking Western
稳定性与储存
Store at 2-8°C long term (12 months). Upon delivery aliquot.
英文名称
BSA Blocking Buffer
储存条件
2-8°C储存
运输条件
低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F0318836 |
分析证书 | B670017 |
| H2412073 |
分析证书 | B670017 |
| ZJ25F0825612 |
分析证书 | B670017 |
| ZJ25F0318835 |
分析证书 | B670017 |
技术文档和文章
Western Blot
目录： 技术文章
Western Blot
目录： 技术文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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危险品化学品经营许可证（带存储）