木瓜凝乳蛋白酶 来源于番木瓜

CAS: 9001-09-6 货号: C1440278 EC号: 232-580-8
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级别和纯度: 天然 ? 天然型(Native)—— 处于天然(非重组、非变性)形态的蛋白/生物分子。当需要天然结构和活性时使用。 EnzymoPure™ ? EnzymoPure™ —— 阿拉丁的高质量酶解决方案系列。当酶纯度和明确活性决定检测或工艺性能时使用。 ≥40,000 IMCU/g enzyme powder
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P14080
生物活性
≥40,000 IMCU/g enzyme powder
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为什么选择此级别

EnzymoPure™,天然 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。

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2-8°C储存,避光,干燥。低温运输 。请查阅批次 COA 获取详细规格。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

  凝乳酶是采用生物工程技术从天然番木瓜未成熟的果实中分离提纯而制得的一种天然生物酶,其分子量为36000属于疏基(—SH)酶。利用凝乳酶可有效凝乳组分生产新鲜的或经短期发酵成熟的干酪,制成新型干酪制品,大大促进国人对干酪的消费,从而促进干酪产业的发展。

产品特点:

1、 凝乳强度高;

2、 蛋白质、脂肪损失少,干酪产率高;

3、 可有效凝乳组分生产新鲜干酪,缩短干酪成熟时间有效控制酪蛋白水解,防止酪蛋白过度水解生成苦味肽;

4、 缓解我国价格较贵的日益紧张的皱胃酶,降低生产成本,具有巨大的社会效益和经济效益。

产品性状:淡黄色或白色固体粉末

应用范围:

1、 食乳制品;

2、 仿制干酪的生产;

3、 适合中国人口味的风味系列化再制干酪;

4、 乳清多肽、含干酪饮料;

5、 医药等行业。

凝乳强度检测:

  凝乳强度的测定采用Arima法,取5ml ,100g/L的脱脂乳,在35℃下保温5min,加入0.5ml,lg/ L的木瓜凝乳酶溶液,快速混合均匀,准确测定从加入酶液到凝固的时间T(s),把40min凝固1ml,100g/ L的脱脂乳的酶量定义为一个索氏单位。

凝乳强度= (2400 / T) × (5 / 0.5) × D(稀释倍数)

使用条件:

凝乳温度:35-95℃,最适宜温度35-37℃,使用时可视具体物料而确定温度值。

使用pH:5-8,其凝乳活性随pH值的降低而增强,凝如速度加快;pH值大于8时,此酶丧失活性。

乳制品的一般使用量:0.1-0.4%,可根据物料及凝乳时间的要求,作适当的增减。

应用案例:

市售脱脂牛奶凝乳工艺:

乳液活化→加酶凝乳→切割→灭酶→过滤→洗涤→脱水→干燥等

关键工艺环节操作:

1、乳液活化:新鲜脱脂牛奶,调节乳液 pH 至 6.1-6.2 之间,添加 0.0-0.16% 左右的氯化钙(CaCl₂),充分搅拌均匀,调节乳温至 39-40℃之间。 

2、凝乳:将凝乳酶(以2000U/g为例)先用少量盐水(约 2% 左右的盐浓度)溶解,加入脱脂牛奶中,迅速搅拌均匀,39-40℃保温,凝乳酶添加量为奶液量的 0.05%(如 10Kg 新鲜脱脂奶液,即添加 1g 凝乳酶),使乳液在 10-20 分钟之内出现凝结。注:凝乳酶添加量与凝乳时间成正比,添加量越多,凝乳时间越快。用户可根据具体要求调节凝乳酶的添加量。 

3、切割:待凝乳切口光滑时,将凝乳切割成 1-3 厘米的小块。 

4、灭酶:切割完成后,升温至 60-65℃,要求每分钟升温 1℃左右,加热 30-1 小时分钟,使凝乳酶失活,同时使凝乳脱水收缩,利于从乳清中分离。

储存:

建议在阴凉干燥的环境下避光保存。

贮藏过久或贮藏条件不利,会使酶活不同程度的降低;如温度湿度过高,则需要在使用时适当的增加使用量。

安全:

凝乳酶属于纯天然的酶制剂,是蛋白质,食用酶制剂的食品如同食用含有蛋白质类食物一般,对人体有益无害。

对于部分敏感人群,如直接摄入高浓缩的酶粉或雾滴,有可能引起过敏,过长时间接触有可能刺激皮肤、眼睛和粘膜组织。在操作过程中建议配戴口罩、眼罩等防护用具,剩余或洒出的酶粉需及时处理,对于大量洒出的酶粉应轻轻扫回容器,少量则用真空吸走或用水浸湿清理。

凝乳酶是一种生物活性物质,易受重金属离子(Fe³⁺, Cu²⁺, Hg⁺, Pb⁺等)和氧化剂的抑制及破坏作用,在贮存或使用过程中应避免与之接触。

  Chymopapain is a natural biological enzyme prepared by separating and purifying from the immature fruits of natural papaya using bioengineering technology. It has a molecular weight of 36,000 and belongs to the thiol (-SH) enzyme family. Using papain can effectively coagulate milk components to produce fresh cheese or cheese matured through short-term fermentation, developing new cheese products. This will greatly promote domestic cheese consumption and further drive the development of the cheese industry.

Product Features

1. High coagulation activity;

2. Low loss of protein and fat, leading to high cheese yield;

3. Effectively coagulates milk components for fresh cheese production, shortens cheese maturation time, controls casein hydrolysis, and prevents bitter peptides from excessive casein hydrolysis;

4. Alleviates the shortage and high cost of chymosin in China, reduces production costs, and delivers significant social and economic benefits.

Product Properties

Pale yellow or white solid powder.

Application Scope

1. Dairy products;

2. Processed cheese production;

3. Series of flavored processed cheeses suitable for Chinese taste preferences;

4. Whey peptides and cheese-containing beverages;

5. Pharmaceutical and other industries.

Determination of Coagulation Activity

The Arima method is adopted for determination. Take 5 ml of 100 g/L skim milk, incubate at 35°C for 5 minutes, add 0.5 ml of 1 g/L papain solution, and mix rapidly and uniformly. Accurately measure the time T (in seconds) from enzyme addition to coagulation. One Soxhlet unit is defined as the amount of enzyme that coagulates 1 ml of 100 g/L skim milk within 40 minutes.

Coagulation Activity = (2400 / T) × (5 / 0.5) × D (Dilution Factor)

Usage Conditions

Coagulation temperature: 35–95°C, optimal temperature 35–37°C. The specific temperature can be determined based on the actual material.

Operating pH: 5–8. Coagulation activity increases with decreasing pH, accelerating coagulation rate. The enzyme loses activity when pH > 8.

General dosage in dairy products: 0.1–0.4%. The dosage can be appropriately adjusted according to material properties and required coagulation time.

Application Case:

Curding Process for Commercial Skimmed Milk:

Emulsion Activation → Enzyme-Added Curdling → Cutting → Enzyme Inactivation → Filtration → Washing → Dehydration → Drying, etc.

Key Process Step Operations:

  1. Emulsion Activation: Take fresh skimmed milk, adjust the emulsion pH to 6.1-6.2, add about 0.0-0.16% calcium chloride (CaCl₂), stir thoroughly until uniform, and adjust the milk temperature to 39-40°C.
  2. Curdling: First dissolve rennet (taking 2000U/g as an example) in a small amount of brine in a small amount of brine (salt concentration ~2%), then add it to the skimmed milk and stir quickly until uniform. Incubate at 39-40°C. The rennet dosage is 0.05% of the milk volume (e.g., add 1g rennet for 10kg fresh skimmed milk), and allow the emulsion to coagulate within 10-20 minutes.Note: The rennet dosage is proportional to the curdling time—the higher the dosage, the faster the curdling. Users can adjust the rennet dosage according to specific requirements.
  3. Cutting: When the curd has a smooth cut surface, cut it into 1-3 cm pieces.
  4. Enzyme Inactivation: After cutting, raise the temperature to 60-65°C at a rate of ~1°C per minute, and heat for 30-60 minutes. This inactivates the rennet, while also dehydrating and shrinking the curd to facilitate separation from whey.

Storage

Store in a cool, dry, and dark environment.Prolonged storage or unfavorable storage conditions will reduce enzyme activity to varying degrees. If temperature and humidity are too high, appropriately increase the dosage during use.

Safety

Papain is a natural enzyme preparation and a type of protein. Foods containing enzyme preparations are as beneficial and harmless to the human body as protein-rich foods.For some sensitive individuals, direct intake of highly concentrated enzyme powder or droplets may cause allergies. Prolonged contact may irritate the skin, eyes, and mucous membranes. It is recommended to wear protective equipment such as masks and goggles during operation. Residual or spilled enzyme powder should be disposed of promptly: large amounts should be gently swept back into the container, and small amounts can be removed with a vacuum or cleaned by wetting with water.Papain is a biologically active substance, easily inhibited or destroyed by heavy metal ions (such as Fe³⁺, Cu²⁺, Hg⁺, Pb⁺) and oxidants. Avoid contact with these substances during storage or use.

规格

产品名称
木瓜凝乳蛋白酶 来源于番木瓜
别名
凝乳酶
英文别名
Papaya proteinase II | PPII
规格或纯度
天然,EnzymoPure™,≥40,000 IMCU/g enzyme powder
生化机理
Cysteine proteinase with a high level of diversity in substrate specificity.
生物活性
≥40,000 IMCU/g enzyme powder
种属
Papaya
来源
天然
CAS编号和信息
9001-09-6
酶学委员会编号
EC 3.4.22.6
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
浓度
≥40,000 IMCU/g enzyme powder
储存条件
2-8°C储存,避光,干燥
运输条件
低温运输
稳定性与储存
长期储存2-8℃(24个月);复溶后建议分装;避光,密封。
单位定义
把40min凝固1ml,100g/ L的脱脂乳的酶量定义为一个索氏单位。

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批号(Lot Number) 证书类型 货号
ZJ25F1230101 分析证书 C1440278
ZJ25F1230100 分析证书 C1440278
ZJ25F1230098 分析证书 C1440278
ZJ25F1230097 分析证书 C1440278
ZJ25F1230096 分析证书 C1440278
ZJ25F1230099 分析证书 C1440278
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常见问题

什么是「天然」?
「天然」表示该物料经过加工和检测,适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如,电子应用的金属含量、电池应用的粒度、光学应用的折射率,或对水分敏感工艺的水分含量。
「EnzymoPure?」是什么?
「EnzymoPure?」是阿拉丁科学的高端产品线之一,专为多个工作流程中的高纯度应用而设计。与同类工作流程中的标准级别相比,「EnzymoPure?」产品通常具有更严格的规格、更严谨的 QC 以及更强的批间一致性。
本产品应如何储存?
请于2–8 °C、避光、干燥条件下保存。请与干燥剂一同密封保存,本品对湿气敏感。
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