BioReagent 级 提供 ≥80%(HPLC) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存,干燥。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
CellTracker is a fluorescent dye well suited for monitoring cell movement or location. After loading into cells, the dye is well retained, allowing for multigenerational tracking of cellular movements.
Cell movement and location studies require specialized probes that are nontoxic to living cells and well retained, allowing for multigenerational tracking. The CellTracker fluorescent probes are available in a range of fluorescent colors to match instrument lasers and filters, and to accommodate co-staining with antibodies or other cell analysis probes. These dyes are excellent tools for monitoring cell movement, location, proliferation, migration, chemotaxis, and invasion.
Features of the CellTracker dyes include:
The CellTracker fluorescent probes have been designed to freely pass through cell membranes; however, once inside the cell are transformed into cell-impermeant reaction products. The CellTracker fluorescence probes (except for CellTracker Deep Red) contain a chloromethyl or bromomethyl group that reacts with thiol groups, utilizing a glutathione S-transferase–mediated reaction. In most cells, glutathione levels are high (up to 10 mM) and glutathione transferase is ubiquitous. CellTracker Deep Red probe contains a succinimidyl ester reactive group, which reacts with amine groups present on proteins.
After conversion to impermeant versions, the CellTracker fluorescent probes are well retained in living cells through several generations. The probes are transferred to daughter cells, but are not transferred to adjacent cells in a population. Cells loaded with the CellTracker fluorescent probes display fluorescence for at least 72 hours and exhibit ideal tracking dye properties—they are stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, several CellTracker fluorescent probes with various excitation and emission spectra are available allowing for multiplexing in fluorescence imaging. The CellTracker dyes are retained with fixation and permeabilization, enabling their use with antibodies for fluorescence immunostaining applications.
Spectral characteristics of the fluorescent CellTracker probes:
Fluorescent CellTracker reagents include the blue-fluorescent chloromethyl derivatives of amino-, hydroxy-, and difluorohydroxycoumarin (CMAC, CMHC and CMF2HC), the green-fluorescent chloromethyl derivatives of fluorescein diacetate (CMFDA) and a BODIPY dye, the orange-fluorescent CMTMR and CMRA, and the red-fluorescent CMTPX. CellTracker Blue CMAC, CMHC, and CMF2HC, CellTracker Violet, the violet-fluorescent bromomethyl derivative of coumarin (BMQC), CellTracker Green BODIPY, CellTracker Orange CMTMR, and CellTracker Red CMTPX do not require enzymatic cleavage to activate their fluorescence, whereas the green CMFDA and orange CMRA do require enzymatic cleavage. The impermeable reaction products of the chloromethyl or bromomethyl coumarins have excellent retention, strong fluorescence, and relatively uniform cytoplasmic staining, making these derivatives potentially useful for correcting motion artifacts in imaging. CMFDA is colorless and non-fluorescent until cytosolic esterases cleave off the acetates, releasing a brightly fluorescent product.
In addition to bright fluorescence and excellent retention, the CellTracker fluorescent probes do not contribute to cytotoxicity.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0232337 |
分析证书 | C1455426 |
危险品化学品经营许可证（带存储）