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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
一氧化碳中毒是含碳物质燃烧不完全时的产物经呼吸道吸入引起的，中毒机理是一氧化碳与血红蛋白的亲合力比氧与血红蛋白的亲合力高上百倍，所以一氧化碳极易与血红蛋白结合，形成碳氧血红蛋白，使血红蛋白丧失携氧的能力和作用造成组织窒息，对全身的组织细胞均有毒性作用，尤其对大脑皮质的影响最为严重。
自备仪器和试剂
1、蒸馏水
2、比色杯或96孔板、分光光度计或酶标仪
操作步骤
1、用蒸馏水稀释Alkaline Buffer (10×) 至1×，密闭保存，待用。
2、准确称取亚硫酸盐若干份，每1份为20mg，待用。
3、取大试管若干支，各加新鲜血液0.1ml，另取一不吸烟的健康人血液0.1ml作为对照，分别加入1×Alkaline Buffer 20ml，混匀。
4、立即分别加入亚硫酸盐20mg，混匀。
6、计算结果
HbCO(%)=｛2.44×(A₅₃₈ / A₅₇₈)-2.68｝×100%
参考区间：
| 正常人 | 0.2%～0.5% |
| 城市居民 | 0.5%～1.5% |
注意事项
1、每次检查应设正常对照。
2、Alkaline Buffer不要被酸碱污染，同时注意密闭保存。
3、为了您的安全和健康，请穿实验服并戴一次性手套操作。
4、试剂开封后请尽快使用，以防影响后续实验效果。
Carbon monoxide poisoning occurs when the incomplete combustion of carbon‑containing materials produces carbon monoxide, which is then inhaled through the respiratory tract. The mechanism of poisoning is that carbon monoxide has an affinity for hemoglobin hundreds of times greater than that of oxygen, allowing it to bind readily with hemoglobin to form carboxyhemoglobin (HbCO). This binding deprives hemoglobin of its oxygen‑carrying capacity and function, leading to tissue hypoxia. Carbon monoxide exerts toxic effects on all tissue cells, with the most severe impact on the cerebral cortex.
Detection Principle:
After carbon monoxide binds to hemoglobin, it forms cherry‑red carboxyhemoglobin (HbCO). HbCO is not reduced by sulfite, whereas oxyhemoglobin (HbO₂) is reduced. HbCO has an absorption peak at 538 nm, and HbO₂ has an absorption peak at 578 nm. The ratio of the two absorbances is used in a formula to calculate the HbCO content. This method is primarily used for the quantitative detection of carboxyhemoglobin (carbon monoxide hemoglobin) in human and animal blood. This kit is intended for research use only and is not suitable for clinical diagnosis or other purposes.
Reagents, consumables and Equipments not provided
1. Distilled water
2. Cuvettes or 96‑well plate, spectrophotometer or microplate reader
Procedure
1.. Dilute the Alkaline Buffer (10×) to 1× with distilled water. Store tightly sealed and set aside for use.
2. Accurately weigh several portions of Sulfite, each portion being 20 mg. Set aside for use.
3. Take several large test tubes and add 0.1 mL of fresh blood to each. Also take 0.1 mL of blood from a healthy non‑smoker as a control. Add 20 mL of 1× Alkaline Buffer to each tube and mix well.
4. Immediately add 20 mg of Sulfite to each tube and mix thoroughly.
5. Transfer an appropriate amount of the above blood mixture into cuvettes or a 96‑well plate. Measure the absorbance at 538 nm (A₅₃₈) and at 578 nm (A₅₇₈) using a spectrophotometer or microplate reader.
Note: Measurements must be completed within 10 minutes.
6. Calculation of Results
HbCO (%) = {2.44 × (A₅₃₈ / A₅₇₈) – 2.68} × 100%
Reference Interval:
| Population | HbCO Level |
| Normal individuals | 0.2%–0.5% |
| Urban residents | 0.5%–1.5% |
Notes
1. Each assay should include a normal control.
2. Avoid contamination of the Alkaline Buffer with acids or alkalis, and store it tightly sealed.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.
4. Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
| C1507809 | Component | 50T | Storage |
| C1507809A | Alkaline Buffer (10×) | 100 mL | RT. |
| C1507809B | 亚硫酸盐 | 2 g | RT. |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0131495 |
分析证书 | C1507809 |
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