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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
哺乳动物CarE，也称脂族酯酶（aliesterase），广泛分布于组织和器官，属于丝氨酸水解酶家族。CarE催化含酯键、酰胺键和硫酯键的内源性与外源性物质水解，但不能催化水解乙酰胆碱及其类似物。CarE参与脂质运输和代谢，并且与多种药物、环境毒物以及致癌物的解毒和代谢有关，有机磷农药可结合并且抑制CarE活性。 测定原理：CarE能催化乙酸-1-萘酯生成萘酯，固蓝显色；在450 nm光吸收增加速率，计算CarE活性。
Carboxylesterase (CarE) in mammals, also known as aliesterase, is widely distributed in tissues and organs and belongs to the serine hydrolase family. CarE catalyzes the hydrolysis of endogenous and exogenous substances containing ester bonds, amide bonds, and thioester bonds, but it does not catalyze the hydrolysis of acetylcholine or its analogs. CarE is involved in lipid transport and metabolism and is associated with the detoxification and metabolism of various drugs, environmental toxins, and carcinogens. Organophosphate pesticides can bind to and inhibit CarE activity. Assay Principle: CarE catalyzes the hydrolysis of 1-naphthyl acetate to produce naphthol, which subsequently reacts with a Fast Blue dye to form a colored product. The increase in absorbance at 450 nm is measured, and the rate of increase is used to calculate CarE activity.
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