计算溶液所需的质量、体积或浓度。
概述
量子点荧光微球主要用于定量侧向层析检测试剂中，兼具长斯托克斯位移、卓越光稳定性和高量子产率等特性，能有效降低背景干扰并提升检测灵敏度。其表面带有羧基修饰，可通过一步法直接与抗体/抗原实现高效偶联。与时间分辨微球相比，量子点微球密度较高，离心步骤简单，可节省生产时间和设备成本。
| 货号 | 荧光颜色 | 微球粒径 | 官能团 | 激发波长 | 发射波长 |
| C1521548-01 | 红色 | 300nm | 羧基 | 300~500nm | 620±10nm |
| C1521548-02 | 绿色 | 300nm | 羧基 | 300~500nm | 520±10nm |
| C1521548-03 | 黄色 | 300nm | 羧基 | 300~500nm | 570±10nm |
量子点微球在 300~500nm(常用 365nm）的激发波长下，发射的光有较大的 stoks 位移以及光稳定性强的特点。不同内核的量子点发色光颜色不同且无光污染现象，在多项目联检的侧向层析检测中，具有更多优势。
建议使用一步法标记
1、 选择合适的标记缓冲液（如 0.02M PB7.4、0.05MMES 5.5 等 ），向标记缓冲液中加入量子点，量子点终浓度为 3mg/ml；
2、 加入一定量的吐温 20，建议终浓度为 0.01%（v/v）。
3、 以上混合液超声混匀后，加入标记抗体，建议添加量为 240μg/ml，混匀后，室温震荡反应 10min.
4、 加入浓度均为 10mg/ml 的活化液 NHS、EDC 溶液，至 NHS 终浓度为 0.4mg/ml，EDC 终浓度0.2mg/ml，混匀后室温震荡反应 2h。
5、 加入与量子点混合液等体积的 5% BSA 溶液，封闭 1h。
6、 在 3500xg 条件下，离心 10min，弃去上清。
7、 复溶液复溶量子点终浓度至 0.4mg/ml。
注意事项
1、缓冲液使用不合适，会出现标记聚集的情况，建议在标记过程中增加超声操作，分散量子点。
2、离心条件随离心的体积发生相应变化。
3、量子点使用之前，需超声分散。
4、强氧化性的条件下，会影响量子点的使用寿命。
5、说明书的使用方法仅为建议使用方法，需根据具体的标记物进行调整。
产品优势
1. 表面功能化：羧基；
2. 荧光稳定性更佳；
3. 荧光微球粒径均一，单分散性优异，稳定性好；
4. 检测灵敏度更高；
5. 适配免疫层析定量检测、微孔板超敏检测两大技术平台。
Quantum dot fluorescent microspheres are mainly used in quantitative lateral flow detection reagents. They feature large Stokes shift, excellent photostability and high quantum yield, which can effectively reduce background interference and improve detection sensitivity. The microspheres are carboxyl-modified on the surface and can be efficiently conjugated with antibodies or antigens directly via a one-step method. Compared with time-resolved microspheres, quantum dot microspheres have higher density, which simplifies centrifugation steps and reduces production time and equipment costs.
| Catalog No. | Fluorescent Color | Microsphere Size | Functional Group | Excitation Wavelength | Emission Wavelength |
| C1521548-01 | Red | 300 nm | Carboxyl | 300~500 nm | 620±10 nm |
| C1521548-02 | Green | 300 nm | Carboxyl | 300~500 nm | 520±10 nm |
| C1521548-03 | Yellow | 300 nm | Carboxyl | 300~500 nm | 570±10 nm |
Under excitation wavelengths ranging from 300 to 500 nm (with 365 nm as the commonly used wavelength), quantum dot microspheres feature a large Stokes shift and outstanding photostability. Quantum dots with different core materials emit distinct fluorescent colors without optical interference, rendering them superior for lateral flow assays involving multiplex simultaneous detection.
One-step labeling protocol is recommended
1. Select an appropriate labeling buffer (e.g., 0.02 M PB, pH 7.4; 0.05 M MES, pH 5.5), and add quantum dots to the buffer to a final concentration of 3 mg/mL.
2. Add an appropriate amount of Tween 20, with a recommended final concentration of 0.01% (v/v).
3. After sonicating the above mixture to homogeneity, add the labeling antibody at a recommended dosage of 240 μg/mL. Mix well and react with shaking at room temperature for 10 min.
4. Add activation solutions of NHS and EDC (both at 10 mg/mL) to achieve final concentrations of 0.4 mg/mL for NHS and 0.2 mg/mL for EDC, respectively. Mix well and react with shaking at room temperature for 2 h.
5. Add an equal volume of 5% BSA solution to the quantum dot mixture and block for 1 h.
6. Centrifuge at 3500×g for 10 min and discard the supernatant.
7. Redissolve the quantum dots in a resuspension buffer to a final concentration of 0.4 mg/mL.
Precautions
1. Inappropriate buffer selection may lead to labeling aggregation. It is recommended to introduce sonication during the labeling process to disperse the quantum dots.
2. Centrifugation conditions should be adjusted accordingly with the centrifugation volume.
3. Quantum dots must be dispersed by sonication prior to use.
4. Strongly oxidizing conditions will compromise the service life of quantum dots.
5. The protocol described in this instruction is for reference only and should be adjusted according to the specific labeling conjugate.
Product Advantages
1.Surface functionalization: carboxyl groups；
2.Better fluorescence stability；
3.Fluorescent microspheres have uniform particle size, excellent monodispersity and good stability；
4.Higher detection sensitivity；
5.Suitable for two major technical platforms: quantitative immunochromatographic detection and microplate ultrasensitive detection.
规格
规格或纯度
粒径300nm, 10mg/ml in H2O
英文名称
Carboxyl fluorescent quantum dot nanospheres
储存条件
2-8°C储存,避光
运输条件
低温运输
名称和识别符
分子类型
未知
技术文档
高级数据
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溶液计算器
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