计算溶液所需的质量、体积或浓度。
钙含量检测试剂盒（邻甲酚酞络合酮, 比色法）
有货
为什么选择此级别
BioReagent,单标法 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
钙（Calcium）是一种金属元素，常温下呈银白色晶体，动物的骨骼、蛤壳、蛋壳都含有碳酸钙。检测生命体钙含量，主要通过检测钙离子浓度实现的，用分光光度法检测钙较为常见，该方法需要合适的金属指示剂或选择性结合钙离子后引起变色的染料化合物，目前较为常用的染料有邻-甲酚酞络合酮（OCPC）、偶氮胂Ⅲ、甲基麝香草酚蓝（MTB）等。
钙含量检测试剂盒（邻甲酚酞络合酮, 比色法）是利用溶液中钙离子在碱性条件下能与OCPC结合，生成紫红色的络合物，加入镁离子螯合剂，去除镁离子背景干扰，通过分光光度计检测575nm处吸光度，根据公式计算出钙含量。
该法的优点：1. 是国际上推荐的血清钙的常规检测方法；2. 试剂单一，操作简便；3. 灵敏度高；4. 显色稳定；5. 使用样本量小。
该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
产品组成：
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自备材料：
1. 离心管或试管、比色杯、分光光度计
操作步骤（仅供参考）：
1. 制备样品
① 血浆、血清样品：血浆、血清按照常规方法制备，可以直接用于该试剂盒的测定，-20℃冻存，用于Ca的检测。
② 细胞或组织样品：取恰当细胞或组织进行匀浆，低速离心取上清，-20℃冻存，用于Ca的检测。
③ 高浓度样品：如果样品中含有较高浓度的Ca，可以使用ddH₂O稀释，不宜使用普通蒸馏水稀释。
④（选做） 样品准备完毕后可以用BCA蛋白浓度测定试剂盒测定蛋白浓度，以便于后续计算单位蛋白重量组织或细胞内的Ca含量。
2. 配制Ca显色工作液
临用前，取钙检测缓冲液和OCPC显色液等量混合，即为Ca显色工作液，即配即用；4℃避光保存，不宜久置。
3. Ca加样
选用经稀盐酸处理及去离子水清洁的干燥试管或者一次性无菌聚乙烯离心管，按照下表设置空白管、标准管、测定管，溶液应按照顺序依次加入，并注意避免产生气泡。如果样品中的钙离子含量过高，可以减少样品用量或适当稀释后再进行测定，样品的检测最好能设置平行管。
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4. Ca测定
混匀，室温静置10min，以蒸馏水调零，比色杯光径1cm，分光光度计575nm处检测，读取标准管、测定管的吸光度（即为A标准，A测定）。
计算：
血清、血浆中钙 (mmol/L) = (A测定 － A空白) ÷ (A标准 － A空白) × 2.5
组织中钙 (mmol/g) = (A测定 － A空白) ÷ (A标准 － A空白) × 2.5 × V ÷ 待测样品质量(g)
细胞中钙 (mmol/g) = (A测定 － A空白) ÷ (A标准 － A空白) × 2.5 ÷ 待测样品蛋白浓度(g/L)
式中：
A测定 = 测定管的吸光度
A标准 = 标准管的吸光度
A空白 = 空白管的吸光度
V = 组织提取液的总体积（L）
参考区间：
健康成年人血清钙浓度：2.1 ~ 2.6 mmol/L
儿童血清钙浓度：2.25 ~ 2.67 mmol/L
性能指标：
线性范围：1.25 ~ 5.0 mmol/L
批内CV：1.08% ~ 1.60%
回收率：98% ~ 101.5%
批间CV：3.05% ~ 4.12%
注意事项：
1. 溶血样本对检测有干扰，尽量避免采用溶血样本。
2. OCPC显色液偶尔会结晶析出，配制成的显色工作液有时会析出，可取上清液使用。
3. 该产品对钙离子敏感度较高，测定器皿一定要干净，最好使用一次性聚乙烯塑料器皿。
4. 可用血清或肝素抗凝血浆样本，不宜采用钙螯合剂及草酸盐做抗凝剂的样本。
5. 胆红素可产生负干扰，含有较高胆红素的样本可用血清对照消除该干扰。
6. 本法能够用于自动生化分析仪终点检测法。
7. 在该试剂盒条件下建议待测样品中钙离子浓度应大于0.5 mmol/L为宜，否则有可能造成检测误差。
8. 如果样品浓度过高，应用蒸馏水稀释后重测，结果乘以稀释倍数。
9. 试剂开封后请尽快使用，以防影响后续实验效果。
有效期：6个月有效。按要求保存。
Calcium is a metallic element that appears as a silvery-white crystal at room temperature. Animal bones, shells, and eggshells all contain calcium carbonate. The detection of calcium content in living organisms is primarily achieved by measuring calcium ion concentration. Spectrophotometric detection of calcium is relatively common; this method requires suitable metal indicators or dye compounds that undergo color changes upon selective binding to calcium ions. Commonly used dyes include o-Cresolphthalein Complexone (OCPC), Arsenazo III, Methyl Thymol Blue (MTB), among others.
The Calcium Content Assay Kit (o-Cresolphthalein Complexone, Colorimetric Method) utilizes the principle that calcium ions bind with OCPC under alkaline conditions to form a purple-red complex. Magnesium ion chelating agents are added to eliminate background interference from magnesium ions. Absorbance is measured at 575 nm using a spectrophotometer, and the calcium content is calculated according to the formula.
Advantages of this method: 1. It is the internationally recommended routine method for serum calcium measurement; 2. Single reagent system, simple operation; 3. High sensitivity; 4. Stable color development; 5. Small sample volume required.
This kit is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
Components
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Materials to Be Prepared by the User
1. Centrifuge tubes or test tubes, cuvettes, spectrophotometer
Operating Procedure (For Reference Only)
1. Sample Preparation
① Plasma and Serum Samples: Plasma and serum are prepared by conventional methods and can be used directly for determination with this kit. Store at -20°C for Ca detection.
② Cell or Tissue Samples: Take appropriate cells or tissues and homogenize. Centrifuge at low speed and collect the supernatant. Store at -20°C for Ca detection.
③ High-Concentration Samples: If the sample contains high concentrations of Ca, dilute with ddH<sub>2</sub>O. Ordinary distilled water should not be used for dilution.
④ (Optional) After sample preparation, protein concentration may be determined using a BCA Protein Assay Kit to facilitate subsequent calculation of Ca content per unit protein weight in tissues or cells.
2. Preparation of Ca Working Color Development Solution
Immediately before use, mix equal volumes of Ca Assay Buffer and OCPC Chromogenic Solution to prepare the Ca Working Color Development Solution. Prepare fresh and use immediately. Store at 4°C protected from light; do not store for extended periods.
3. Ca Loading
Use dry test tubes treated with dilute hydrochloric acid and cleaned with deionized water, or disposable sterile polyethylene centrifuge tubes. Set up blank tube, standard tube, and sample tube according to the table below. Solutions should be added sequentially in order, taking care to avoid bubble formation. If the calcium ion content in the sample is too high, reduce the sample volume or dilute appropriately before measurement. It is recommended to set up duplicate tubes for sample testing.
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4. Ca Measurement
Mix well and let stand at room temperature for 10 minutes. Zero the spectrophotometer with distilled water. Use a cuvette with a 1 cm light path. Measure absorbance at 575 nm. Record the absorbances of the standard tube and sample tube (designated as Astandard and Asample).
Calculation
Serum and plasma calcium (mmol/L) = (Asample – Ablank) ÷ (Astandard – Ablank) × 2.5
Calculation
Serum and plasma calcium (mmol/L) = (Asample – Ablank) ÷ (Astandard – Ablank) × 2.5
Cellular calcium (mmol/g) = (Asample – Ablank) ÷ (Astandard – Ablank) × 2.5 ÷ protein concentration of test sample (g/L)
Where:
Asample = absorbance of the sample tube
Astandard = absorbance of the standard tube
Ablank = absorbance of the blank tube
V = total volume of tissue extraction solution (L)
Precautions
Healthy adult serum calcium: 2.1 – 2.6 mmol/L
Pediatric serum calcium: 2.25 – 2.67 mmol/L
Performance Characteristics
Linear range: 1.25 – 5.0 mmol/L
Intra-assay CV: 1.08% – 1.60%
Recovery rate: 98% – 101.5%
Inter-assay CV: 3.05% – 4.12%
Precautions
1. Hemolyzed samples interfere with the assay; avoid using hemolyzed samples whenever possible.
2. OCPC Chromogenic Solution may occasionally crystallize, and the prepared working color development solution may sometimes precipitate. The supernatant can be used for testing.
3. This product has high sensitivity to calcium ions. All measurement vessels must be clean; disposable polyethylene plastic vessels are recommended.
4. Serum or heparin-anticoagulated plasma samples may be used. Samples containing calcium chelating agents or oxalate anticoagulants should not be used.
5. Bilirubin may cause negative interference. Samples with high bilirubin content can be corrected by using a serum blank to eliminate this interference.
6. This method can be adapted for endpoint detection on automated biochemical analyzers.
7. Under the conditions of this kit, it is recommended that the calcium ion concentration in the test sample be greater than 0.5 mmol/L to avoid measurement errors.
8. If the sample concentration is too high, dilute with distilled water and re-measure. Multiply the result by the dilution factor.
9. Once opened, use the reagents promptly to prevent compromising subsequent experimental results.can be adapted for endpoint detection on automated biochemical analyzers.
Shelf Life: 6 months. Store as instructed.
规格
规格或纯度
BioReagent, 单标法
英文名称
Calcium Content Assay Kit (o-Cresolphthalein Complexone, Colorimetric Method)
应用
细胞代谢, 钙离子检测
样品类型
组织, 细胞, 血浆, 血清
检测方法
分光光度法
定量&半定量
定量
检测仪器
可见光分光光度计
检测波长
575nm
敏感性
Light-sensitive
储存与运输
储存条件
2-8°C储存,避光
运输条件
低温运输
稳定性与储存
Store at 2-8℃ long term (6 months). Store in the dark.
组分与储存
| 组分 | 名称 | 物理外观 | 100T | Storage |
| C1521764A | 钙标准 (2.5mmol/L) | 液体 | 1ml | 2-8℃. |
| C1521764B | 钙检测缓冲液 | 液体 | 50ml | 2-8℃. 避光 |
| C1521764C | OCPC 显色液 | 液体 | 50ml | 2-8℃. 避光 |
| C1521764D | ddH₂O | 液体 | 10ml | 2-8℃. |
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高级数据
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838432 |
分析证书 | C1521764 |
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