BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蔗糖转化酶（Invertase，Ivr）不可逆地水解蔗糖为果糖和葡萄糖，是高等植物蔗糖代谢的关键酶之一。根据最适pH，高等植物Ivr分为酸性转化酶（Acid invertase，AI）和中性转化酶（Neutral invertase，NI）。AI的最适pH为3~5，并进一步分为可溶性AI（S-AI）和细胞壁结合型不溶性AI（B-AI）两种形式。B-AI定位于细胞间隙并与细胞壁结合，其主要功能是参与韧皮部质外体卸载过程中的蔗糖分解，以维持源与库之间的蔗糖浓度梯度。在本试剂盒中，B-AI催化蔗糖降解产生还原糖，后者与3,5-二硝基水杨酸反应生成棕红色氨基化合物，该化合物在510 nm处具有特征吸收峰。在一定范围内，510 nm处吸光值的增加速率与B-AI活性成正比。
Sucrose invertase (Invertase, Ivr) irreversibly hydrolyzes sucrose into fructose and glucose and is one of the key enzymes in sucrose metabolism in higher plants. Based on its optimal pH, Ivr in higher plants is classified into acid invertase (AI) and neutral invertase (NI). The optimal pH for AI ranges from 3 to 5, and it is further divided into two forms: soluble AI (S-AI) and cell wall-bound insoluble AI (B-AI). B-AI is localized in the intercellular space and bound to the cell wall. Its primary function is to participate in the breakdown of sucrose during apoplastic unloading in the phloem, thereby maintaining the sucrose concentration gradient between source and sink tissues. In this assay kit, B-AI catalyzes the degradation of sucrose to produce reducing sugars, which then react with 3,5-dinitrosalicylic acid to form a reddish-brown amino compound. This compound exhibits a characteristic absorption peak at 510 nm. Within a certain range, the rate of increase in absorbance at 510 nm is proportional to B-AI activity.
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