计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肉毒碱棕榈酰转移酶是存在于线粒体内膜的一类酰基转移酶。可逆地催化从酰基辅酶A将酰基转移至L-肉毒碱的反应,在转运脂肪酸通过线粒体内膜的过程中起重要作用。 肉碱脂酰转移酶(CPT-I)催化肉碱和脂酰辅酶A在丙二酰辅酶A存在与否的条件下,生成 脂酰肉碱,并释放出巯基辅酶A(COA-SH),与Ellman试剂DN-TB反应后,产生黄色的TNB,通过测定412nm处的光吸收,来计算出CPT-I活性。
Carnitine palmitoyltransferase is a class of acyltransferases located in the inner mitochondrial membrane. It reversibly catalyzes the transfer of an acyl group from acyl-CoA to L-carnitine, playing a crucial role in transporting fatty acids across the inner mitochondrial membrane. Carnitine palmitoyltransferase I (CPT-I) catalyzes the reaction between carnitine and fatty acyl-CoA, with or without malonyl-CoA, to form acylcarnitine and release coenzyme A (CoA-SH). The released CoA-SH reacts with Ellman's reagent (DTNB) to produce yellow TNB. The CPT-I activity is then calculated by measuring the absorbance at 412 nm.