计算溶液所需的质量、体积或浓度。
肌酸是一种内源性化合物,其功能是通过肌酸激酶的磷酸化使机体维持高的ATP/ADP比率。肌酸补充剂是一种运动性能增强剂,亦用于治疗肌肉和神经退行性等疾病。因此肌酸的检测在研究和开发中具有广泛的应用。 本试剂盒利用肌酸酶特异作用于肌酸生成肌氨酸,肌氨酸在肌氨酸氧化酶的作用下生成过氧化氢,过氧化氢与显色剂反应呈现(粉)红色,该有色物质在 520nm 有最大吸收峰,进而计算得到肌酸含量。
Creatine is an endogenous compound that functions to maintain a high ATP/ADP ratio in the body through phosphorylation by creatine kinase. Creatine supplements are used as exercise performance enhancers and also for the treatment of muscle and neurodegenerative diseases. Therefore, creatine detection has broad applications in research and development. This kit utilizes creatinase to specifically act on creatine to produce sarcosine. Sarcosine is then acted on by sarcosine oxidase to generate hydrogen peroxide, which reacts with a chromogenic agent to produce a (pink) red color. This colored substance has a maximum absorption peak at 520 nm, and the creatine content is calculated accordingly.