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几丁质主要存在于虾、蟹、昆虫等甲壳类动物的外壳与软体动物的器官(例如乌贼的软骨),以及真菌类的细胞壁中。而几丁质酶(EC 3.2.1.14)可催化几丁质水解,具有抵御真菌侵染的作用,成为抗真菌病害的研究热点。 测定原理:几丁质酶水解几丁质产生N-乙酰氨基葡萄糖,进一步与对二甲氨基苯甲醛产生红色化合物,在585nm处有特征吸收峰,吸光值增加速率反映了几丁质酶的活性。
Chitin is mainly found in the exoskeletons of crustaceans such as shrimp, crabs, and insects, in the organs of mollusks (e.g., the cartilage of squid), as well as in the cell walls of fungi. Chitinase (EC 3.2.1.14) catalyzes the hydrolysis of chitin and plays a role in defending against fungal infection, making it a research focus in antifungal disease control. Determination Principle: Chitinase hydrolyzes chitin to produce N-acetylglucosamine, which further reacts with p-dimethylaminobenzaldehyde to form a red compound with a characteristic absorption peak at 585 nm. The rate of increase in absorbance reflects chitinase activity.