本系列细胞冻存液围绕不同层级科研需求分层研发，共 C1524464、C1524465、C1524466、C1524467、C1524468 五款型号，全部采用低浓度 DMSO 配方，从经济型基础科研到高端定制、肿瘤细胞专用全覆盖，适配绝大多数常用实验细胞系。
| 项目号 | 产品名称 | 核心成分
| 优势
| 适用细胞系 | C1524464
| 细胞冻存液 (通用型基础款)
| 低浓度DMSO、无蛋白、无血清
| 成本低，低浓度DMSO， 无蛋白、无血清。 | L929、B16、K562、HeLa、DC2.4、Molt-4、THP-1 等
| C1524465
| 细胞冻存液 (通用型进阶款)
| 低浓度DMSO、无蛋白
| 性价比高，低DMSO、无蛋白，复苏率≥90%。
| L929、B16、K562、HeLa、DC2.4、THP-1、293T 等
| C1524466
| 细胞冻存液 (通用型旗舰A款)
| 低浓度DMSO、优化配比蛋白
| 优化蛋白与DMSO，复苏率≥95%，增殖快。
| HT22、L929、Hep-G2、B16、K562、HeLa、DC2.4、THP-1、bmdm、NK、pbmc、PK15、CHO 等
| C1524467
| 细胞冻存液 (通用型旗舰B款)
| 低浓度DMSO、优化配比蛋白
| 侧重保护细胞结构，减少细胞器损伤，提升功能。 | HT22、L929、Hep-G2、B16、B16-F10、K562、HeLa、LLC-OVA、DC2.4、4T1、MCF-7、Molt-4、H9C2、THP-1 等
| C1524468
| 细胞冻存液 (肿瘤细胞专用款)
| 低浓度DMSO、含血清
| 含血清与低DMSO，维持肿瘤细胞稳定。
| HT22、Hep-G2、B16-F10、HeLa、DC2.4、4T1、H9C2、293T 等 |
|
主要成分：氨基酸、无机盐、冷冻保护剂。
产品定位：通用型基础款，适用于多种细胞，满足不同场景需求，保障细胞复苏后的功能性。 性能稳定，高性价比。
预期用途：适用于各类细胞冷冻保存，冻存后的细胞仅能用于科研实验或体外分析检测，不能用于临床治疗。
使用说明：本品在灌装前已进行灭菌处理，无需稀释即可使用；细胞培养应按照相应规程进行。
细胞冻存规程：
1.收集对数增长期细胞，离心（1000 rpm，5 min）后去上清。
2.加入完全培养基重悬后进行细胞计数以及活率检测，离心后去上清（如不需要计数看细胞初始状态，此步骤可省略）。
3.使用本产品重悬（轻柔吹打，避免气泡），推荐冻存密度为1×10⁶-1×10⁷个/mL，冻存体积0.5-1.5mL/管。
4.将分装好的细胞冻存管，在4℃孵育20 min后转入-80℃冰箱过夜，次日投入液氮保存即可。
注意：
[1]本公司产品在使用前应提前在4℃冰箱预冷，避免保护剂因温度过高对细胞造成损伤。
[2]为避免复苏过程中冻存管炸裂，投入液氮之前确保冻存管完全密封。
细胞复苏规程：
1.将含有细胞的冻存管从液氮中取出，迅速置于37℃水浴锅中快速融化。
2.将解冻的细胞转入加有等体积完全培养基的无菌离心管后，离心（1000 rpm，5 min）弃上清液，使用完全培养基重悬细胞。
3.将上述细胞悬液转入加有完全培养基的细胞培养瓶中，混匀后置于细胞培养箱中培养。
注意：复苏后立即检测可能不够准确，贴壁后或4-48h（根据细胞生长特性而定）再进行检测更为准确。
注意事项：
1.收到产品后请立即转移到4℃冰箱中保存。
2.产品使用过程中如接触皮肤或眼睛请立即用水冲洗，若有不适请及时就医。
3.如本产品放置后出现浑浊、沉淀等变质问题，请勿使用并立即联系技术人员。
4.为避免造成细胞污染，请在生物安全柜中开启瓶盖。
This series of cell cryopreservation solutions is developed in tiers to meet diverse research requirements and consists of five catalog numbers: C1524464, C1524465, C1524466, C1524467 and C1524468. All formulations contain low-concentration DMSO, covering applications from cost-effective basic research to high-end customized formulations and tumor cell-specific cryopreservation, compatible with the vast majority of commonly used laboratory cell lines.
Item No.
| Product Name
| Core Components
| Advantages
| Objectives
| Applicable Cell Lines
| C1524464
| Cell Freezing Medium (General Basic)
| Low-concentration DMSO, Protein-Free, Serum-Free
| Low cost; low DMSO concentration; protein-free and serum-free.
| Deliver economical cell cryopreservation solutions for basic scientific research.
| L929、B16、K562、HeLa、DC2.4、Molt-4、THP-1, etc.
| C1524465
| Cell Freezing Medium (General Advanced)
| Low-Concentration DMSO, Protein-Free
| High cost performance, low DMSO, protein-free; post-thaw cell viability ≥90%.
| Supply cost-effective solutions for research scenarios with tight budget constraints.
| L929、B16、K562、HeLa、DC2.4、THP-1、293T, etc. | C1524466
| Cell Freezing Medium (Universal Flagship Type A)
| Low-Concentration DMSO, Optimally Formulated Protein
| Optimized matching of protein and DMSO; post-thaw viability ≥95%, rapid cell proliferation.
| Provide high-performance cryopreservation solutions for cutting-edge research and high-end pharmaceutical applications.
| HT22、L929、Hep-G2、B16、K562、HeLa、DC2.4、THP-1、bmdm、NK、pbmc、PK15、CHO, etc.
| C1524467
| Cell Freezing Medium (Universal Flagship Type B)
| Low-concentration DMSO, Optimally Formulated Protein
| Focuses on cellular structure protection, alleviates organelle damage, improves cellular function.
| Offer precise and efficient cell cryopreservation solutions for specialized research and pharmaceutical development.
| HT22、L929、Hep-G2、B16、B16-F10、K562、HeLa、LLC-OVA、DC2.4、4T1、MCF-7、Molt-4、H9C2、THP-1, etc.
| C1524468
| Cell Freezing Medium (Tumor Cell Specific)
| Low-Concentration DMSO, Serum-Containing
| Formulated with serum and low DMSO to stabilize tumor cells.
| Launch dedicated cell cryopreservation products for tumor research studies.
| HT22、Hep-G2、B16-F10、HeLa、DC2.4、4T1、H9C2、293T, etc. |
|
Main components: amino acids, inorganic salts, cryoprotectants.
Product Positioning: General-purpose basic formulation applicable to multiple cell types to accommodate various experimental scenarios and preserve cellular functionality post-thaw. Featuring stable performance and excellent cost performance.
Intended Use: For cryopreservation of various cell types. Cryopreserved cells are limited to research experiments or in vitro analytical assays and shall not be used for clinical therapy.
Instructions for Use: Sterilized prior to filling; ready-to-use without dilution. Cell culture shall be performed in accordance with standard operating protocols.
Cell Cryopreservation Protocol:
1. Harvest cells in logarithmic growth phase, centrifuge at 1000 rpm for 5 min and discard the supernatant.
2. Resuspend cell pellet in complete culture medium for cell counting and viability assessment, followed by centrifugation and supernatant removal. This step may be omitted if cell quantification is unnecessary and initial cell condition is confirmed.
3. Resuspend cells with this product via gentle pipetting to avoid air bubbles. Recommended seeding density: 1×10⁶ to 1×10⁷ cells per mL; fill volume per cryovial: 0.5–1.5 mL.
4. Incubate sealed cryovials at 4 °C for 20 min, transfer to a −80 °C freezer for overnight storage, then immerse in liquid nitrogen the following day for long-term preservation.
Notes:
[1] Pre-chill the product at 4 °C before use to prevent cell damage induced by overheated cryoprotectants.
[2] Ensure complete tight sealing of cryovials before liquid nitrogen immersion to prevent tube rupture during cell thawing.
Cell Thawing Protocol:
1. Retrieve cryovials from liquid nitrogen and rapidly thaw in a 37 °C water bath.
2. Transfer thawed cell suspension into a sterile centrifuge tube pre-filled with an equal volume of complete medium, centrifuge at 1000 rpm for 5 min, discard supernatant and resuspend cells in fresh complete medium.
3. Seed the cell suspension into a cell culture flask containing complete medium, mix thoroughly and incubate in a cell culture incubator.
Notes: Post-thaw cell detection performed immediately often yields inaccurate results; testing is recommended after cell attachment or within 4–48 hours post-recovery, adjusted according to inherent growth characteristics of the target cell line.
General Precautions:
1. Store the product at 4 °C immediately upon receipt.
2. In case of skin or ocular contact during handling, rinse thoroughly with running water and seek prompt medical attention if irritation occurs.
3. Discard deteriorated product showing turbidity or precipitation after storage and contact technical support immediately.
4. Open the product cap inside a biosafety cabinet to avoid microbial contamination of cell samples.
ZJ26F1039643
危险品化学品经营许可证（带存储）