计算溶液所需的质量、体积或浓度。
细胞冻存液 (通用型旗舰B款)
有货
为什么选择此级别
BioReagent,无菌,用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本系列细胞冻存液围绕不同层级科研需求分层研发，共 C1524464、C1524465、C1524466、C1524467、C1524468 五款型号，全部采用低浓度 DMSO 配方，从经济型基础科研到高端定制、肿瘤细胞专用全覆盖，适配绝大多数常用实验细胞系。
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主要成分：氨基酸、无机盐、白蛋白、冷冻保护剂。
产品定位： 通用型旗舰B款，为创新研发端提供精准高效冻存方案，细胞适用范围、产品稳定性进一步提升，对细胞功能的保障性更高。
预期用途：适用于各类细胞冷冻保存，冻存后的细胞仅能用于科研实验或体外分析检测，不能用于临床治疗。
使用说明：本品在灌装前已进行灭菌处理，无需稀释即可使用；细胞培养应按照相应规程进行。
细胞冻存规程：
1.收集对数增长期细胞，离心（1000 rpm，5 min）后去上清。
2.加入完全培养基重悬后进行细胞计数以及活率检测，离心后去上清（如不需要计数看细胞初始状态，此步骤可省略）。
3.使用本产品重悬（轻柔吹打，避免气泡），推荐冻存密度为1×10⁶-1×10⁷个/mL，冻存体积0.5-1.5mL/管。
4.将分装好的细胞冻存管，在4℃孵育20 min后转入-80℃冰箱过夜，次日投入液氮保存即可。
注意：
[1]本公司产品在使用前应提前在4℃冰箱预冷，避免保护剂因温度过高对细胞造成损伤。
[2]为避免复苏过程中冻存管炸裂，投入液氮之前确保冻存管完全密封。
细胞复苏规程：
1.将含有细胞的冻存管从液氮中取出，迅速置于37℃水浴锅中快速融化。
2.将解冻的细胞转入加有等体积完全培养基的无菌离心管后，离心（1000 rpm，5 min）弃上清液，使用完全培养基重悬细胞。
3.将上述细胞悬液转入加有完全培养基的细胞培养瓶中，混匀后置于细胞培养箱中培养。
注意：复苏后立即检测可能不够准确，贴壁后或4-48h（根据细胞生长特性而定）再进行检测更为准确。
注意事项：
1.收到产品后请立即转移到4℃冰箱中保存。
2.产品使用过程中如接触皮肤或眼睛请立即用水冲洗，若有不适请及时就医。
3.如本产品放置后出现浑浊、沉淀等变质问题，请勿使用并立即联系技术人员。
4.为避免造成细胞污染，请在生物安全柜中开启瓶盖。
This series of cell cryopreservation solutions is developed in tiers to meet diverse research requirements and consists of five catalog numbers: C1524464, C1524465, C1524466, C1524467 and C1524468. All formulations contain low-concentration DMSO, covering applications from cost-effective basic research to high-end customized formulations and tumor cell-specific cryopreservation, compatible with the vast majority of commonly used laboratory cell lines.
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Main Components: Amino acids, inorganic salts, albumin, cryoprotectants.
Product Positioning: Universal Flagship Grade B. It delivers precise and efficient cryopreservation solutions for innovative R&D, featuring broader cell compatibility and enhanced product stability, as well as superior protection of cellular functions.
Intended Use: For cryopreservation of various cell types. Cryopreserved cells are limited to research experiments or in vitro analytical assays and shall not be used for clinical therapy.
Instructions for Use: Sterilized prior to filling; ready-to-use without dilution. Cell culture shall be performed in accordance with standard operating protocols.
Cell Cryopreservation Protocol:
1. Harvest cells in logarithmic growth phase, centrifuge at 1000 rpm for 5 min and discard the supernatant.
2. Resuspend cell pellet in complete culture medium for cell counting and viability assessment, followed by centrifugation and supernatant removal. This step may be omitted if cell quantification is unnecessary and initial cell condition is confirmed.
3. Resuspend cells with this product via gentle pipetting to avoid air bubbles. Recommended seeding density: 1×10⁶ to 1×10⁷ cells per mL; fill volume per cryovial: 0.5–1.5 mL.
4. Incubate sealed cryovials at 4 °C for 20 min, transfer to a −80 °C freezer for overnight storage, then immerse in liquid nitrogen the following day for long-term preservation.
Notes:
[1] Pre-chill the product at 4 °C before use to prevent cell damage induced by overheated cryoprotectants.
[2] Ensure complete tight sealing of cryovials before liquid nitrogen immersion to prevent tube rupture during cell thawing.
Cell Thawing Protocol:
1. Retrieve cryovials from liquid nitrogen and rapidly thaw in a 37 °C water bath.
2. Transfer thawed cell suspension into a sterile centrifuge tube pre-filled with an equal volume of complete medium, centrifuge at 1000 rpm for 5 min, discard supernatant and resuspend cells in fresh complete medium.
3. Seed the cell suspension into a cell culture flask containing complete medium, mix thoroughly and incubate in a cell culture incubator.
Notes: Post-thaw cell detection performed immediately often yields inaccurate results; testing is recommended after cell attachment or within 4–48 hours post-recovery, adjusted according to inherent growth characteristics of the target cell line.
General Precautions:
1. Store the product at 4 °C immediately upon receipt.
2. In case of skin or ocular contact during handling, rinse thoroughly with running water and seek prompt medical attention if irritation occurs.
3. Discard deteriorated product showing turbidity or precipitation after storage and contact technical support immediately.
4. Open the product cap inside a biosafety cabinet to avoid microbial contamination of cell samples.
规格
别名
细胞冻存液 | 通用细胞冻存液 | 细胞系冷冻保存液
英文别名
Cell Cryopreservation Medium | Cell Freezing Medium
规格或纯度
BioReagent, 用于细胞培养, 无菌, 低浓度DMSO、优化配比蛋白
稳定性与储存
Store at 2-8°C long term (12 months).
英文名称
Cell Freezing Medium (Universal Flagship Type B)
储存条件
2-8°C储存
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0738003 |
分析证书 | C1524467 |
| ZJ26F0738004 |
分析证书 | C1524467 |
| ZJ26F0738005 |
分析证书 | C1524467 |
技术文档和文章
溶液计算器
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