0.2 w/v的CHAPS已用于通过脑灌注去除脑内皮。2％(w/v) CHAPS已与硫脲和尿素一起在蛋白质提取中用作再水化缓冲液。10 mM CHAPS已与0.15 M NaCl, Tris–Cl, pH 7.6, EDTA一起在Western Blot分析中用作裂解缓冲液。CHAPS已在未成熟哺乳动物CNS的原位杂交方法中用作杂交缓冲液。
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.A solution at 50 mg/ml in water yields a clear-colorless solution. Aqueous solutions of CHAPS can be autoclaved.0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion. 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction. 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis. CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS.
G2326025
危险品化学品经营许可证（带存储）