计算溶液所需的质量、体积或浓度。
Cy2
有货
为什么选择此级别
Em:510nm,Ex:492nm 级 提供 ≥98% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Cyanines are formally compounds with two nitrogen atoms linked by an odd number of methene units. 26 28 The nitrogen atoms are parts of the heterocyclic units (such as indole, benzoxazol, or benzothiazol). The structures and optical properties of representative cyanine dyes used for in vivo imaging are presented. Cyanines are characterized by long wavelength, tunable absorption and emission, very high extinction coefficient (up to 300,000 M 1 cm 1), good water solubility, and relatively straightforward synthesis .
In Vitro
1.Protein Preparetion 1) In order to obtain the best labeling effect, please prepare the protein (antibody) concentration as 2 mg/mL. 2) The pH value of protein solution shall be 8.5±0.5. If the pH is lower than 8.0, 1m sodium bicarbonate shall be used for adjustment. 3) If the protein concentration is lower than 2 mg/ml, the labeling efficiency will be greatly reduced. In order to obtain the best labeling efficiency, it is recommended that the final protein concentration range is 2-10 mg/mL. 4) The protein must be in the buffer without primary amine (such as Tris or glycine) and ammonium ion, otherwise the labeling efficiency will be affected. 2.Dye Preparation (Example for CY3-NHS ester) Add anhydrous DMSO into the vial of CY3-NHS ester to make a 10 mM stock solution. Mix well by pipetting or vortex. 3.Calculation of dye dosage The amount of CY3-NHS ester required for reaction depends on the amount of protein to be labeled, and the optimal molar ratio of CY3-NHS ester to protein is about 10. Example : assuming the required marker protein is 500 μL 2 mg/mL IgG (MW=150,000), use 100 μL DMSO dissolve 1 mg CY3-NHS ester, the required CY3-NHS ester volume is 5.05 μL, and the detailed calculation process is as follows: 1) mmol (IgG) = mg/mL (IgG) ×mL (IgG) / MW (IgG) =2 mg/mL × 0.5 mL / 150,000 mg/mmol= 6.7×10 -6 mmol\n2) mmol (CY3-NHS ester) = mmol (IgG) × 10 = 6.7×10 -6 mmol×10 = 6.7 × 10 -5 mmol\n3) μL (CY3-NHS ester) = mmol (CY3-NHS ester) ×MW (CY3-NHS ester) / mg/μL (CY3-NHS ester) = 6.7 ×10 -5 mmol ×753.88 mg/mmol / 0.01 mg/μL = 5.05 μL (CY3-NHS ester) 4.Run conjugation reaction 1) A good volume of freshly prepared 10 mg/mL CY3-NHS ester is slowly added to 0.5 mL protein sample In solution, gently shake to mix, then centrifuge briefly to collect the sample at the bottom of the reaction tube. Don'tmix well to prevent protein samples from denaturation and inactivation. 2) The reaction tubules were placed in a dark place and incubated gently at room temperature for 60 minutes at intervals.For 10-15 minutes, gently reverse the reaction tubules several times to fully mix the two reactants and raise the bar efficiency. 5.Purify the conjugation The following protocol is an example of dye-protein conjugate purification by using a Sephadex G-25 column. 1) Prepare Sephadex G-25 column according to the manufacture instruction. 2) Load the reaction mixture (From \Run conjugation reaction\) to the top of the Sephadex G-25 column. 3) Add PBS (pH 7.2-7.4) as soon as the sample runs just below the top resin surface. 4) Add more PBS (pH 7.2-7.4) to the desired sample to complete the column purification. Combine the fractions that contain the desired dye-protein conjugate. Attention: 1.CY dyesis sensitive to light and humidity. Immediately addCY dyesrsolution and discard the unused part. 2. Low concentrations of sodium azide (≤3 mM or 0.02%) or thiomersal (≤0.02 mM or 0.01%) did not significantly interfere with protein labeling; However, 20-50% glycerol will reduce labeling efficiency. 3. Avoid buffering with primary amines (e.g., Tris, glycine) or ammonium ions,Itcompete with labeled proteins. 4. This product is only for scientific research by professionals, and shall not be used in clinical diagnosis or treatment, food or medicine. 5. For your safety and health, please wear lab coat and disposable gloves. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Form:Solid
规格
规格或纯度
Ex:492nm, Em:510nm, ≥98%
英文名称
Cy2
生化机理
从形式上讲，氰基化合物是由奇数个甲烯单元连接两个氮原子的化合物。26 28 氮原子是杂环单元（如吲哚、苯并恶唑或苯并噻唑）的一部分。代表化合物的结构和光学性质
储存条件
避光,-20°C储存
运输条件
超低温运输
纯度
≥98%
Ex/Em(nm)
Ex:492nm;Em:510nm
名称和识别符
分子类型
小分子
Smiles
CC[N+]1=C(/C=C/C=C(N2CCCCCC([O-])=O)/OC3=C2C=CC=C3)OC4=CC=CC=C41
分子量
418.49
技术文档
高级数据
化学和物理性质
溶解性
DMSO : ≥ 30 mg/mL (71.69 mM)
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品的纯度是多少？
本产品的化学纯度（含量）为 ≥98%。批次的具体数值以分析证书（COA）为准。
「Em:510nm」表示什么？
「Em:510nm」是我们目录中用于筛选和描述某些试剂的产品属性，并非具有独立规格的标准级别。它最常用于标识适用于荧光标记和检测的产品。
本产品应如何储存？
请于?20 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
「Ex:492nm」表示什么？
「Ex:492nm」是我们目录中用于筛选和描述某些试剂的产品属性，并非具有独立规格的标准级别。它最常用于标识适用于荧光标记和检测的产品。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 260430-02-2，分子式为 C25H26N2O4，分子量为 418.49 g/mol。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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