计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥99% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
CAM833 is a potent orthosteric inhibitor of the interaction between BRCA2 and RAD51 with a K d of 366 nM against the ChimRAD51 protein. CAM833 also inhibits RAD51 oligomerization. CAM833 increases the progression of G2/M-arrested cells into apoptosis
In Vitro
CAM833 (3.125-50 μM; 24 h) causes a concentration-dependent decrease in RAD51 foci and subsequent increase in DNA damage. CAM833 (25 μM) inhibits RAD51 molecular clustering at DNA damage sites and suppresses extended RAD51 filament assembly. CAM833 (0-50 μM) inhibits DNA repair by homologous recombination. CAM833 (20 μM; 0-72 h) potentiates radiation-induced cell-cycle arrest and increases apoptosis over time in HCT116 cells. CAM833 (0.1-100 μM; 96 h) causes a dose-dependent growth inhibition of multiple cancer-derived human cell lines that is enhanced when combined with ionizing radiation. CAM833 (20 μM; 96 h) potentiates the growth suppressive effect of PARP1 inhibition in BRCA2 wild-type cells. CAM833 (96 h) alone inhibits the growth of HCT116 colon carcinoma cells with a GI 50 (50% growth inhibition) of 38 μM, when combined with 3 Gy IR, CAM833 suppresses the growth of HCT116 cells with a GI 50 of 14 μM. The quinoline of CAM833 occupies a hotspot, the Phe-binding pocket on RAD51 and the methyl of the substituted α-methylbenzyl group occupies the Ala-binding pocket. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Cycle AnalysisCell Line: HCT116 cells Concentration: 20 μM Incubation Time: 0-72 h Result: In the control the percentage of cells in the apoptotic subG1 fraction remains below 5% throughout, in the compound-treated cells this rises progressively to peak at 15% at 48 hours.
Form:Solid
IC50& Target:K d : 355 nM (ChimRAD51, measured by FP), 366 nM (ChimRAD51, measured by ITC)
规格
规格或纯度
≥99%
英文名称
CAM833
生化机理
CAM833 是 BRCA2 和 RAD51 之间相互作用的强效正交抑制剂，对 ChimRAD51 蛋白的 K d 值为 366 nM。CAM833 还能抑制 RAD51 的寡聚化。CAM833 可增加 G2/M 停滞细胞进入凋亡的进程
储存条件
-20°C储存
运输条件
超低温运输
作用类型
抑制剂
纯度
≥99%
名称和识别符
分子类型
小分子
分子量
528.96
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 125 mg/mL (236.31 mM; Need ultrasonic)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
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