计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥99% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,干燥。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Cyclic-di-GMP diammonium is a STING agonist and a bacterial second messenger that coordinates different aspects of bacterial growth and behavior, including motility, virulence, biofilm formation, and cell cycle progression. Cyclic-di-GMP diammonium has anti-cancer cell proliferation activity and also induces elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP diammonium can be used in cancer research
In Vitro
Cyclic-di-GMP diammonium (0.5-50 μM; 5 days) inhibits proliferation of human colon cancer cells. ?\nCyclic-di-GMP diammonium (0.5-50 μM; 5 days) specifically elevates CD4 expression in Jurkat cells. ?\nCyclic-di-GMP diammonium (0.5-50 μM; 5 days) induces cell cycle arrest at the S-phase in Jurkat cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation AssayCell Line: H508 cells Concentration: 0.5-50 µM Incubation Time: 5 days Result: Reduced basal H508 cell proliferation by approx 15%, even inhibited acetylcholine- and EGF-induced cell proliferation. Cell Viability AssayCell Line: Jurkat cells Concentration: 50 µM Incubation Time: 24 h Result: Specifically induced of CD4 (no effect on the expression of CD8), with a 6.3-fold upregulation over control and in a dose-dependent manner. Cell Cycle AnalysisCell Line: Jurkat cells Concentration: 50 µM Incubation Time: 24 h Result: Increased the percentage of cells in S-phase by 79%, with almost complete disappearance of G2/M-phase cells which decreased by 93%.
In Vivo
Cyclic-di-GMP diammonium (100 μg/per; i.v.; two sequential vaccinations 9 days apart) enhances TriVax-induced immune responses to melanoma in mice and further increased the anti-tumor effects of TriVax. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: C57BL/6 (B6) mice (8- to 10-week-old). Dosage: 100 µg/per Administration: Intravenous injection; two sequential vaccinations 9 days apart; combine with TriVax. Result: Significantly higher numbers of antigen-specific CD8 T cells when combined with TriVax. (TriVax consisted of a mixture of 120 μg Pam-hgp100, 100 µg hgp100 or 100 µg Ova, 50 or 25 μg anti-CD40 antibody, and 25 μg Poly-IC). Enhanced the anti-tumor activity of TriVax.
Form:Solid
IC50& Target:STING
规格
别名
环二鸟苷酸二铵
规格或纯度
≥99%
英文名称
Cyclic-di-GMP diammonium
生化机理
环二-GMP 二氨是一种 STING 激动剂和细菌第二信使，可协调细菌生长和行为的不同方面，包括运动、毒力、生物膜形成和细胞周期进展。环二-GMP 二铵盐对细菌的生长和繁殖具有促进作用。
储存条件
-20°C储存,干燥
运输条件
超低温运输
作用类型
激活剂
纯度
≥99%
名称和识别符
分子类型
小分子
分子量
724.47
技术文档
高级数据
化学和物理性质
溶解性
H2O : 50 mg/mL (69.02 mM; Need ultrasonic)
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
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