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伴刀豆球蛋白A(con A)是从刀豆中精制的植物凝集素。它是一种四聚体蛋白,有四个相同的28kDa亚基。Con A具有连接多种糖蛋白的甘露糖残基的能力,并且可以刺激淋巴细胞。
质量
通过特定亲和层析法进行高度纯化
分析说明
据报道,凝集活性以μg/ml表示,并且在Ca2+ 和Mn2+ 浓度为1 mg/ml、pH 6.8的磷酸缓冲盐溶液中进行连续稀释测定。该活性是在25°C下孵育1小时的2%人红细胞悬浮液可以凝集的最低浓度。
用途
来自刀豆(Canavalia ensiformis)的伴刀豆球蛋白A已被用于:
作为T淋巴细胞有丝分裂原在淋巴细胞增殖测定中确定与有机氯污染物(OC)的相关性
用于体外刺激T细胞杂交瘤、 BW5147细胞和原代CD4+ T细胞进行T细胞疫苗接种和肝炎的诱导
在抑制小鼠T细胞增殖试验中刺激小鼠脾细胞
在有丝分裂原测定中刺激脾脏
Concanavalin A (con A) is a plant lectin refined from jack beans. It is a tetrameric protein, which has four identical 28 kDa subunits. Con A has the ability to connect with the mannose residues of several glycoproteins and can stimulate lymphocytes.
Quality
Highly purified by specific affinity chromatography
Analysis Note
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Purpose
Concanavalin A from Canavalia ensiformis (Jack bean) has been used:
as T-lymphocyte mitogen in lymphocyte proliferation assay for correlations with organochlorine contaminants (OCs)
for in vitro stimulation of T cell hybridomas, BW5147 cells and primary CD4+ T cells for T cell vaccination and for induction of hepatitis
for the stimulation of mice splenocytes in the suppression of T-lymphocytes proliferation assay
for the stimulation of spleen in mitogen assays