Creatinine Deiminase (CNI, EC 3.5.4.21) is a hydrolase that specifically catalyzes the ring-opening hydrolysis of creatinine, producing N-methylhydantoin and ammonia (NH₃). This enzyme is commonly found in microorganisms and is widely used in clinical diagnostics for the detection of creatinine levels. It serves as a key enzymatic component in vitro diagnostic reagents for renal function assessment.

Reaction

Properties

Source: Microorganism
Isoelectric point: 5.8
Michaelis constant: 6.6×10^-5 M (Creatinine)
Optimum pH: 8.0                     Fig. 1
Optimum temperature: 40~55℃                     Fig. 3
pH Stability: 7.5~9.5 (30℃, 20hr)                     Fig. 2
Thermal stability: <60℃ (pH 7.5, 60min)                     Fig. 4
Inhibitors: Co2+, Cu2+, Fe3+, Ni2+, Zn2+, SDS
Effect of various chemicals:                     Table 1

规格

产品名称

肌酐脱氨酶 (CNI)

别名

肌氨酸酐脱亚胺酶 | 肌酐脱亚胺酶 | 肌酐亚胺水解酶 | 肌酐水解酶

英文别名

Creatinine deaminase | Creatinine iminohydrolase | Creatinine hydrolase

规格或纯度

生物活性,重组,ActiBioPure™,高性能,EnzymoPure™,≥20 U/mg enzyme powder; ≥100 U/mg protein

生物活性

≥20 U/mg enzyme powder; ≥100 U/mg protein

表达系统

E. coli

来源

重组表达

SDS-PAGE

46 kDa (SDS-PAGE)

CAS编号和信息

37289-15-9

酶学委员会编号

EC 3.5.4.21

分子类型

酶

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

浓度

≥20 U/mg enzyme powder; ≥100 U/mg protein

储存条件

避光,-20°C储存,干燥,避免反复冻融

运输条件

超低温运输

稳定性与储存

Store at 4℃ short term (15 days). Store at -20℃ long term (2 years). Upon delivery aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

单位定义

One unit will cause the formation of one micromole of ammonia per min at pH 7.5 at 37℃.

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ25F0825177	分析证书	C774080
ZJ25F0825176	分析证书	C774080
ZJ25F0825175	分析证书	C774080
ZJ25F0825174	分析证书	C774080

技术文档和文章

c774080_creatinine deiminase cni product manual.pdf

c774080 肌酐脱氨酶 cni 说明书.pdf

肌酐酶法检测的反应原理、方法体系与结果质量控制

目录：技术文章

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Creatininase-Based Creatinine Detection: Reaction Principles, Method Systems, and Result Quality Control

目录：技术文章

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Creatinine Content Detection: Main Methods, Reaction Principles, and Result Control

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肌酐代谢相关酶的催化机制及其在肾功能检测中的应用

目录：技术文章

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Catalytic Mechanisms of Creatinine Metabolism-Related Enzymes and Their Applications in Renal Function Testing

目录：技术文章

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溶液计算器