本试剂盒采用可以特异性结合病毒DNA/RNA的离心吸附柱和独特的缓冲液系统，适用于 从200 μl血清/血浆/拭子/全血/组织匀浆液等样本中提取病毒的DNA/RNA。离心吸附柱中采用 的硅基质材料为本公司特有新型材料，高效、专一吸附DNA/RNA，可最大限度去除杂质蛋白 等。提取的病毒纯度高，质量稳定可靠，可适用于各种常规操作，包括qPCR、RT-qPCR、 PCR和文库构建等实验。
产品组分表：
| D1128032 | Component | 50 T | Storage | | D1128032A | Buffer MLA | 30 ml | RT | | D1128032B | Buffer RLA | 30 ml | RT | | D1128032C | Buffer MWP | 30 ml | RT | | D1128032D | RNase-Free ddH₂O | 15 ml | RT | | D1128032E | 吸附柱WHT | 50 个 | RT | | D1128032F | 2 ml Collection Tubes | 50 个 | RT |
|
储存条件
该试剂盒置于常温干燥条件下，可保存15个月。
注意事项
1. 将样品平衡至常温。
2. 所有操作步骤，若无特别指出，均在常温下进行。
3. 操作前必须做好各种防御措施。
操作步骤
1. 吸取500 μl Buffer MLA 到1.5 ml RNase-Free离心管中，再加入200 μl样本 (样品需平衡 至常温)，涡旋振荡30 sec混匀。
2. 将步骤1裂解后的样本全部转移至吸附柱WHT中（吸附柱放在收集管中），盖上管盖， 12,000 rpm离心30 sec，弃废液，将吸附柱放回收集管中。
3. 加入500 μl Buffer RLA，盖上管盖，12,000 rpm离心30 sec，弃废液，将吸附柱放回收 集管。
4. 加入500 μl Buffer MWP，盖上管盖，12,000 rpm 离心30 sec，弃废液，将吸附柱放回收 集管。
5. 12,000 rpm离心1 min，弃废液。
6. 将吸附柱放入一个1.5 ml离心管中，打开盖子，向吸附膜中间部位悬空滴加30-50 μl RNase-Free ddH2O，盖上盖子，12,000 rpm离心30 sec收集核酸，立即进行实验或长期 保存于-20℃。
物理外观：Liquid
是否无菌：否
This kit adopts spin‑columns capable of specifically binding viral DNA/RNA together with a unique buffer system, and is suitable for the extraction of viral DNA/RNA from 200 μL samples such as serum, plasma, swabs, whole blood and tissue homogenate. The silica‑based material used in the spin‑column is a proprietary novel material of our company, which can adsorb DNA/RNA efficiently and specifically and remove impurity proteins to the maximum extent. The extracted viral nucleic acid features high purity and stable, reliable quality, and is compatible with various routine downstream procedures, including qPCR, RT‑qPCR, PCR, library construction and other experiments.
Composition Table:
| D1128032 | Component | 50 T | Storage | | D1128032A | Buffer MLA | 30 ml | RT | | D1128032B | Buffer RLA | 30 ml | RT | | D1128032C | Buffer MWP | 30 ml | RT | | D1128032D | RNase-Free ddH₂O
| 15 ml | RT | | D1128032E | Spin Column WHT
| 50 个 | RT | | D1128032F | 2 ml Collection Tubes | 50 个 | RT |
|
Storage Conditions:
This kit can be stored for 15 months at room temperature in a dry environment.
Precautions:
1. Equilibrate samples to room‑temperature.
2. Unless otherwise specified, all operation steps shall be performed at room temperature.
3. Appropriate protective measures must be taken before operation.
Operating Procedures:
1. Pipette 500 μL of Buffer MLA into a 1.5 mL RNase‑Free centrifuge tube, then add 200 μL sample (the sample shall be equilibrated to room temperature). Vortex for 30 sec to mix thoroughly.
2. Transfer all the lysed sample from Step 1 into Spin Column WHT (place the spin‑column inside the collection tube). Close the cap, centrifuge at 12,000 rpm for 30 sec. Discard the waste liquid and place the spin‑column back into the collection tube.
3. Add 500 μL of Buffer RLA, close the cap, centrifuge at 12,000 rpm for 30 sec. Discard the waste liquid and place the spin‑column back into the collection tube.
4. Add 500 μL of Buffer MWP, close the cap, centrifuge at 12,000 rpm for 30 sec. Discard the waste liquid and place the spin‑column back into the collection tube.
5. Centrifuge at 12,000 rpm for 1 min, then discard the waste liquid.
6. Transfer the spin‑column into a new 1.5 mL centrifuge tube. Open the cap, and add 30‑50 μL RNase‑Free ddH₂O drop‑wise to the centre of the adsorption membrane without touching the membrane. Close the cap, centrifuge at 12,000 rpm for 30 sec to elute nucleic acid. Proceed with downstream experiments immediately or store long‑term at −20 °C.
ZJ25F0724829
危险品化学品经营许可证（带存储）