Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.

Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.
DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes.

规格

产品名称

脱氧核糖核酸酶Ⅱ 来源于猪脾(纯化,溶液)

别名

DNase II;Deoxyribonucleate 3′-oligonucleotido-hydrolase

英文别名

DNase II;Deoxyribonucleate 3′-oligonucleotido-hydrolase

规格或纯度

EnzymoPure™, ≥12,000 units/mg protein

生化机理

该酶是一种溶酶体酸水解酶，在没有金属离子的酸性条件下，可将 DNA 水解为 3′-磷酸寡核苷酸。它是一种非共价连接的α-β异二聚体，分子质量约为 45,000 Da。

CAS编号和信息

9025-64-3

分子类型

酶

储存与运输

浓度

≥12,000 units/mg protein

储存条件

-20°C储存

运输条件

超低温运输

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到1个结果

批号(Lot Number)	证书类型	货号
F1509062	分析证书	D128605

技术文档和文章

CRISPR-Cas9和靶向基因组编辑

目录：技术文章

探索主题:

核酸酶细胞和分子生物学研究靶向效率脱靶突变

CUT&RUN-seq实验方案

目录：实验方案（Protocols）

探索主题:

核酸酶 ChIP CUT&RUN ChIC DNA-蛋白质互作 MNase 刀豆球蛋白A ConA磁珠

RNA提取和反转录实验方案

目录：实验方案（Protocols）

探索主题:

RNA提取逆转录反转录 TRIzol 细胞RNA DNase RNase

RNA Extraction and Reverse Transcription Protocol

目录：实验方案（Protocols）

探索主题:

TRIzol DNase RNase RNA extraction Reverse transcription Cellular RNA

脱氧核糖核酸酶（DNase）：DNA水解切割特性、方法学价值与多场景应用

目录：技术文章

探索主题:

DNase DNA水解磷酸二酯键二价金属离子切割深度去DNA污染降黏度 DNase footprinting DNase-seq NETs

Deoxyribonuclease (DNase): DNA Hydrolytic Cleavage Properties, Methodological Value, and Multi-Context Applications

目录：技术文章

探索主题:

DNase DNase footprinting DNase-seq NETs DNA hydrolysis phosphodiester bond divalent metal ions cleavage depth DNA decontamination viscosity reduction

细胞核酸代谢与调控酶在基因表达、RNA稳态与免疫防御中的功能体系综述

目录：技术文章

探索主题:

质量控制先天免疫核酸代谢核酸调控酶基因表达 RNA稳态 RNA加工 RNA降解 RNA编辑核酸感知

A Functional Systems Review of Cellular Nucleic Acid Metabolism and Regulatory Enzymes in Gene Expression, RNA Homeostasis, and Immune Defense

目录：技术文章

探索主题:

RNA degradation Quality control innate immunity nucleic acid metabolism nucleic acid regulatory enzymes gene expression RNA homeostasis RNA processing RNA editing nucleic acid sensing

核酸酶在核酸样本处理、污染控制与分子实验质控中的应用

目录：技术文章

探索主题:

核酸酶 Rt-qPCR MNase DNase RNase NGS qPCR Benzonase 核酸样本处理 DNA污染控制 RNA污染控制分子实验质控核酸降解

Application of Nucleases in Nucleic Acid Sample Processing, Contamination Control, and Molecular Experiment Quality Control

目录：技术文章

探索主题:

Nuclease Rt-qPCR MNase DNase RNase NGS qPCR Benzonase nucleic acid sample processing DNA contamination control RNA contamination control molecular experiment quality control nucleic acid degradation

CRISPR/Cas9 and targeted genome editing

目录：技术文章

探索主题:

Nuclease Cell and Molecular Biology Research Targeting efficiency Off target mutation

CUT&RUN-seq protocol

目录：实验方案（Protocols）

探索主题:

Nuclease ChIP CUT&RUN ChIC MNase DNA-protein interaction Concanavalin A ConA magnetic beads

此产品的引用文献

如何在您的发表作品中引用阿拉丁的产品和网站? 引用文献检索

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

📚 按应用领域分类引用文献

萃取与后处理溶剂1

有机合成——反应介质1

查看全部 1 篇引用 →

需要帮助选择级别？

我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。

查看 EnzymoPure™ 级别指南 →

大包装与定制包装 需要更大包装或定制规格？请联系我们的团队获取专属报价。

脱氧核糖核酸酶Ⅱ 来源于猪脾(纯化,溶液)

储存与运输

质量文档

文献证明

概述

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

📚 按应用领域分类引用文献

需要帮助选择级别？

查看相关“主题”系列