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DHAR存在于细胞质、线粒体和叶绿体中。DHAR催化GSH还原DHA生成AsA和GSSG，调控细胞AsA/DHA比值，是抗坏血酸-谷胱甘肽氧化还原循环的关键酶。提高植物体内的 DHAR 活性，可提高植物食品中AsA含量，进而提高植物食品的营养品质。 测定原理：DHAR催化GSH还原DHA生成AsA，通过测定DHA减少速率，计算DHAR活性。
Dehydroascorbate reductase (DHAR) is present in the cytoplasm, mitochondria, and chloroplasts. DHAR catalyzes the reduction of dehydroascorbic acid (DHA) by reduced glutathione (GSH) to produce ascorbic acid (AsA) and oxidized glutathione (GSSG). It regulates the cellular AsA/DHA ratio and is a key enzyme in the ascorbate-glutathione redox cycle. Increasing DHAR activity in plants can elevate the AsA content in plant-based foods, thereby enhancing their nutritional quality. Assay Principle: DHAR catalyzes the reduction of DHA by GSH to generate AsA. The activity of DHAR is calculated by measuring the rate of decrease in DHA.
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