脱氧核糖核酸酶I（DNase I）是一种非特异性核酸内切酶，大多数来源于重组 E. coli 菌株，含有牛胰腺 DNase I 的 MBP 融合克隆。DNase I 可用于降解单链或双链 DNA，其原理为 DNase I 水解磷酸二酯键产生带有 5′-磷酸基团和 3′-OH 的单核苷酸或寡核苷酸。Mg²⁺ 或 Mn²⁺ 都可以激活 DNase I 的活性，而 Ca²⁺ 浓度直接影响酶的活性。Mg²⁺ 存在时可在双链 DNA 的每条单链上随机产生切口；而在 Mn²⁺ 存在下可使双链 DNA 断裂，使 DNA 片段化。
Ribonuclease A 简称 RNase A，中文名为核糖核酸酶 A，用于消化 RNA，可用于质粒和基因组抽提等相关实验中去除 RNA。
DNase/RNase 混合液又称 DNA 酶和 RNA 酶混合溶液，由 DNase I、RNase A、Tris-HCl、防腐剂等组成，其中 DNase I 浓度为 1 mg/mL，RNase A 浓度为 0.5 mg/mL，用于去除 DNA、RNA 污染。
该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤（仅供参考）：
根据不同实验，应加入适量的酶，以便充分消化 DNA 和 RNA。
注意事项：
1.应注意避免污染和反复冻融。
2.为了您的安全和健康，请穿实验服并戴一次性手套操作。
3.试剂开封后请尽快使用，以防影响后续实验效果。
Deoxyribonuclease I (DNase I) is a non‑specific endonuclease, mostly derived from recombinant *E. coli* strains with an MBP‑fusion clone of bovine‑pancreatic DNase I. DNase I degrades both single‑stranded and double‑stranded DNA by hydrolyzing phosphodiester bonds to generate mononucleotides or oligonucleotides with 5′‑phosphate and 3′‑OH termini. Its activity can be activated by either Mg²⁺ or Mn²⁺, while Ca²⁺ concentration directly modulates enzymatic performance. In the presence of Mg²⁺, random nicks are introduced into each strand of double‑stranded DNA; in the presence of Mn²⁺, double‑stranded DNA undergoes complete cleavage and fragmentation.
Ribonuclease A (RNase A) digests RNA and is widely applied to remove RNA contamination during plasmid and genomic DNA extraction.
DNase/RNase Mix, also known as DNase‑RNase mixed solution, consists of DNase I, RNase A, Tris‑HCl, preservatives and other components. The final concentrations are 1 mg/mL for DNase I and 0.5 mg/mL for RNase A. This reagent is used to eliminate DNA and RNA contamination in research samples.
For research‑use only. Not intended for clinical diagnosis or any other clinical applications.
Procedure (for reference only):
Add an appropriate amount of the enzyme mixture according to experimental requirements to achieve sufficient digestion of DNA and RNA.
Precautions:
1. Avoid reagent contamination and repeated freeze‑thaw cycles.
2. Wear laboratory coat and disposable gloves for personal safety during handling.
3. Use the reagent promptly after opening to prevent compromised experimental performance.
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